Anti-Cytochrome b245 Light Chain/p22-phox抗体(ab191512)
Key features and details
- Rabbit polyclonal to Cytochrome b245 Light Chain/p22-phox
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Cytochrome b245 Light Chain/p22-phox抗体
参阅全部 Cytochrome b245 Light Chain/p22-phox 一抗 -
描述
兔多克隆抗体to Cytochrome b245 Light Chain/p22-phox -
宿主
Rabbit -
特异性
ab191512 detects endogenous levels of Cytochrome b245 Light Chain/p22-phox protein. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Cytochrome b245 Light Chain/p22-phox.
Database link: P13498 -
阳性对照
- MCF7, NIH3T3 and PC12 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purity is > 95% (by SDS-PAGE) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Predicted molecular weight: 21 kDa.
Blocking in milk is not recommended. |
说明 |
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WB
1/500. Predicted molecular weight: 21 kDa. Blocking in milk is not recommended. |
靶标
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相关性
Cytochrome b245 Light Chain/p22phox is a critical component of the membrane-bound oxidase complex that generates superoxide. It complexes with NOX3 to form a functional NADPH oxidase. -
细胞定位
Cell Membrane -
数据库链接
- Entrez Gene: 1535 Human
- Entrez Gene: 13057 Mouse
- Entrez Gene: 79129 Rat
- Omim: 608508 Human
- SwissProt: P13498 Human
- SwissProt: Q61462 Mouse
- SwissProt: Q62737 Rat
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别名
- CYBA antibody
- Cytochrome b 245 alpha polypeptide antibody
- Cytochrome b 245 light chain antibody
see all
图片
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All lanes : Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab191512)
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CYBA knockout HEK-293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CYBA antibody (ab191512) staining at 1 µg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab191512 was shown to bind specifically to CYBA. A band was observed at 23 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in CYBA knockout cell line ab266721 (knockout cell lysate ab257405). To generate this image, wild-type and CYBA knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab191512)
Lane 1 : MCF7 whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Predicted band size: 21 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab191512 被引用在 4 文献中.
- Yue Y et al. Intrarenal arterial administration of human umbilical cord-derived mesenchymal stem cells effectively preserved the residual renal function of diabetic kidney disease in rat. Stem Cell Res Ther 13:186 (2022). PubMed: 35526048
- Yang H et al. Luteolin induces mitochondrial apoptosis in HT29 cells by inhibiting the Nrf2/ARE signaling pathway. Exp Ther Med 19:2179-2187 (2020). PubMed: 32104282
- Gao X et al. Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro. Front Pharmacol 11:533809 (2020). PubMed: 33071778
- Zhang Y et al. Taurine Attenuates Calpain-2 Induction and a Series of Cell Damage via Suppression of NOX-Derived ROS in ARPE-19 Cells. Oxid Med Cell Longev 2018:4596746 (2018). PubMed: 30151070