重组Anti-Cystatin A抗体[EPR6941] (ab128948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6941] to Cystatin A
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cystatin A抗体[EPR6941]
参阅全部 Cystatin A 一抗 -
描述
兔单克隆抗体[EPR6941] to Cystatin A -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human Cystatin A aa 50-150 (C terminal). The exact sequence is proprietary.
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阳性对照
- NIH 3T3, A549, A431, K562 cell lysates; fetal brain, fetal heart, fetal kidney and Human spleen tissue lysates; NIH3T3 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6941 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128948于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa). |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
This is an intracellular thiol proteinase inhibitor. -
序列相似性
Belongs to the cystatin family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1475 Human
- Entrez Gene: 209294 Mouse
- Entrez Gene: 288075 Rat
- Omim: 184600 Human
- SwissProt: P01040 Human
- SwissProt: P56567 Mouse
- SwissProt: P01039 Rat
- Unigene: 518198 Human
see all -
别名
- AREI antibody
- cstA antibody
- Cystatin A antibody
see all
图片
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All lanes : Anti-Cystatin A antibody [EPR6941] (ab128948) at 1/1000 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Fetal brain tissue lysate
Lane 6 : Fetal heart tissue lysate
Lane 7 : Fetal kidney tissue lysate
Lane 8 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
ab128948 at a 1/10 dilution staining Cystatin A in permeabilized NIH 3T3 cells by intracellular flow cytometry (red), with Rabbit IgG control (green).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab128948 被引用在 1 文献中.
- Komura T et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . PubMed: 28898495