参阅全部 CYR61/CCN1 一抗
经测试应用适用于: ICC/IF, IHC-FoFr, IHC-P, IHC-Fr, WBmore details
种属反应性与反应: Mouse, Rat, Cow, Human
Synthetic peptide corresponding to Mouse CYR61/CCN1. Synthetic peptide surrounding amino acid 166 of mouse Cyr61. The peptide was 10-15 amino acids long.
(Peptide available as
- Jurkat cell lysate, mouse small intestine lysate
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab24448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50. PubMed: 19077055|
|IHC-FoFr||Use at an assay dependent concentration. PMID 17724216|
|IHC-P||Use a concentration of 2 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21490242|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42 kDa).|
功能Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
序列相似性Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
All lanes : Anti-CYR61/CCN1 antibody (ab24448) at 1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse small intestine tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
ICC/IF image of ab24448 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma cells labeling CYR61/CCN1 with ab24448 at 1/100 dilution. Cells were fixed with paraformaldehyde and blocked with 0.05% triton-X100. The cells were blocked with 3% BSA for 1 hour at 20°C followed by incubation with ab24448 in TBS/Tween-20 + 1% BSA for 2 hours at 20°C. A Donkey anti-rabbit Cy3® secondary antibody was used at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence analysis of human synovial fibroblasts labeling CYR61/CCN1 with ab24448 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with methanol. The cells were blocked with 1% BSA for 1 hour at 25°C. The cells were incubated with PBS with ab24448 in 1% BSA for 2 hours at 25°C. A goat anti-rabbit Cy5® secondary antibody was used.
ab24448 staining Human myometrium. Staining can be observed in the cytoplasm and nuclear compartments.
Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
This product has been referenced in:
- Li Z et al. Transplantation of human endometrial perivascular cells with elevated CYR61 expression induces angiogenesis and promotes repair of a full-thickness uterine injury in rat. Stem Cell Res Ther 10:179 (2019). Read more (PubMed: 31215503) »
- Nilsson D et al. Foxc2 is essential for podocyte function. Physiol Rep 7:e14083 (2019). Read more (PubMed: 31062503) »