Key features and details
- Rabbit polyclonal to CYR61/CCN1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 CYR61/CCN1 一抗
经测试应用适用于: IHC-P, WBmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Mouse CYR61/CCN1. Synthetic peptide surrounding amino acid 166 of mouse Cyr61. The peptide was 10-15 amino acids long.
(Peptide available as
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab24448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42 kDa).|
功能Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
序列相似性Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
All lanes : Anti-CYR61/CCN1 antibody (ab24448) at 1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse small intestine tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
ab24448 staining Human myometrium. Staining can be observed in the cytoplasm and nuclear compartments.
Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab24448 被引用在 24 文献中.
- Li Z et al. Transplantation of human endometrial perivascular cells with elevated CYR61 expression induces angiogenesis and promotes repair of a full-thickness uterine injury in rat. Stem Cell Res Ther 10:179 (2019). PubMed: 31215503
- Nilsson D et al. Foxc2 is essential for podocyte function. Physiol Rep 7:e14083 (2019). PubMed: 31062503
- Zaidi ARS et al. Molecular signatures for CCN1, p21 and p27 in progressive mantle cell lymphoma. J Cell Commun Signal 13:421-434 (2019). PubMed: 30465121
- Li X et al. Targeting cysteine-rich angiogenic inducer-61 by antibody immunotherapy suppresses growth and migration of non-small cell lung cancer. Exp Ther Med 16:730-738 (2018). PubMed: 30116327
- Tang C et al. Transcriptional Co-activator Functions of YAP and TAZ Are Inversely Regulated by Tyrosine Phosphorylation Status of Parafibromin. iScience 1:1-15 (2018). PubMed: 30227954