重组Anti-CYP7B1抗体[EPR8395] (ab138497)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8395] to CYP7B1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CYP7B1抗体[EPR8395]
参阅全部 CYP7B1 一抗 -
描述
兔单克隆抗体[EPR8395] to CYP7B1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CYP7B1 aa 400-500. The exact sequence is proprietary.
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阳性对照
- PC3, Human fetal liver, and Human fetal kidney lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数(KD)
KD = 4.10 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8395 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab138497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). |
靶标
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组织特异性
Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine. -
通路
Lipid metabolism; bile acid biosynthesis. -
疾病相关
Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9420 Human
- Omim: 603711 Human
- SwissProt: O75881 Human
- Unigene: 667720 Human
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别名
- 25 hydroxycholesterol 7 alpha hydroxylase antibody
- 25-hydroxycholesterol 7-alpha-hydroxylase antibody
- CP7B antibody
see all
图片
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All lanes : Anti-CYP7B1 antibody [EPR8395] (ab138497) at 1/1000 dilution
Lane 1 : PC3 lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab138497 被引用在 10 文献中.
- Li M et al. Gut microbiota-bile acid crosstalk contributes to the rebound weight gain after calorie restriction in mice. Nat Commun 13:2060 (2022). PubMed: 35440584
- Yang C et al. Flaxseed Powder Attenuates Non-Alcoholic Steatohepatitis via Modulation of Gut Microbiota and Bile Acid Metabolism through Gut-Liver Axis. Int J Mol Sci 22:N/A (2021). PubMed: 34639207
- Evangelakos I et al. Oxysterol 7-α Hydroxylase (CYP7B1) Attenuates Metabolic-Associated Fatty Liver Disease in Mice at Thermoneutrality. Cells 10:N/A (2021). PubMed: 34685636
- Serquiña AKP et al. 25-Hydroxycholesterol Inhibits Kaposi's Sarcoma Herpesvirus and Epstein-Barr Virus Infections and Activates Inflammatory Cytokine Responses. mBio 12:e0290721 (2021). PubMed: 34781692
- Wei M et al. A dysregulated bile acid-gut microbiota axis contributes to obesity susceptibility. EBioMedicine 55:102766 (2020). PubMed: 32408110
- Kuang J et al. Anti-Adipogenic Effect of Theabrownin Is Mediated by Bile Acid Alternative Synthesis via Gut Microbiota Remodeling. Metabolites 10:N/A (2020). PubMed: 33238385
- Huang F et al. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Nat Commun 10:4971 (2019). PubMed: 31672964
- Hauser S et al. mRNA as a Novel Treatment Strategy for Hereditary Spastic Paraplegia Type 5. Mol Ther Methods Clin Dev 15:359-370 (2019). PubMed: 31828178
- Pathak P & Chiang JYL Sterol 12a-Hydroxylase Aggravates Dyslipidemia by Activating the Ceramide/mTORC1/SREBP-1C Pathway via FGF21 and FGF15. Gene Expr 19:161-173 (2019). PubMed: 30890204
- Donepudi AC et al. Deficiency of cholesterol 7a-hydroxylase in bile acid synthesis exacerbates alcohol-induced liver injury in mice. Hepatol Commun 2:99-112 (2018). PubMed: 29404516