Anti-CYP2R1抗体(ab137634)
Key features and details
- Rabbit polyclonal to CYP2R1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CYP2R1抗体
参阅全部 CYP2R1 一抗 -
描述
兔多克隆抗体to CYP2R1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Monkey -
免疫原
Recombinant fragment corresponding to a region within amino acids 83 and 360 of Human CYP2R1.
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阳性对照
- HeLa whole cell lysate; methanol-fixed HeLa cells; FaDu xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137634于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
靶标
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功能
Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). -
疾病相关
Defects in CYP2R1 are the cause of rickets vitamin D-dependent type 1B (VDDR1B) [MIM:600081]; also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 120227 Human
- Entrez Gene: 244209 Mouse
- Omim: 608713 Human
- SwissProt: Q6VVX0 Human
- SwissProt: Q6VVW9 Mouse
- Unigene: 371427 Human
- Unigene: 108037 Mouse
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别名
- CP2R1_HUMAN antibody
- CYP2R1 antibody
- Cytochrome P450 2R1 antibody
see all
图片
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Anti-CYP2R1 antibody (ab137634) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS Page -
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, labelling CYP2R1 using ab137634 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labelling CYP2R1 using ab137634 at 1/200 dilution. lower panel represents cells costained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab137634 被引用在 3 文献中.
- Anisiewicz A et al. Vitamin D Metabolite Profile in Cholecalciferol- or Calcitriol-Supplemented Healthy and Mammary Gland Tumor-Bearing Mice. Nutrients 12:N/A (2020). PubMed: 33172201
- Bukuroshi P et al. Strategies and limitations associated with in vitro characterization of vitamin D receptor activators. Biochem Pharmacol 155:547-561 (2018). PubMed: 30028992
- Bergadà L et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014). PubMed: 24732451