重组Anti-CYP2C9抗体[EPR7340] (ab150364)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7340] to CYP2C9
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CYP2C9抗体[EPR7340]
参阅全部 CYP2C9 一抗 -
描述
兔单克隆抗体[EPR7340] to CYP2C9 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CYP2C9 aa 450-550 (C terminal). The exact sequence is proprietary.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7340 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150364于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa). |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1559 Human
- Omim: 601130 Human
- SwissProt: P11712 Human
- Unigene: 282624 Human
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别名
- (R)-limonene 6-monooxygenase antibody
- (S)-limonene 6-monooxygenase antibody
- (S)-limonene 7-monooxygenase antibody
see all
图片
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All lanes : Anti-CYP2C9 antibody [EPR7340] (ab150364) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with His-tagged empty vector, whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with His-tagged CYP2C9 expression vector, whole cell lysate
Lane 3 : Human liver lysate
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used a loading control for GAPDH.
Exposure time:
Lanes 1,2: 3 seconds
Lanes 3,4: 10 secondsWB result showed an additional band at 37kDa in HepG2 cell lysate, we are unsure as to identify of it.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab150364 被引用在 2 文献中.
- Wang P et al. The Long Noncoding RNA Hepatocyte Nuclear Factor 4α Antisense RNA 1 Negatively Regulates Cytochrome P450 Enzymes in Huh7 Cells via Histone Modifications. Drug Metab Dispos 49:361-368 (2021). PubMed: 33674270
- Zhang X et al. A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. Int J Mol Med 39:101-112 (2017). PubMed: 27959388