Anti-CYP21A2抗体(ab232701)
Key features and details
- Rabbit polyclonal to CYP21A2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CYP21A2抗体
参阅全部 CYP21A2 一抗 -
描述
兔多克隆抗体to CYP21A2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Mouse CYP21A2 aa 1-300. (Expressed in E.coli).
Database link: P03940 -
阳性对照
- IHC-P: Mouse testis and kidney tissues. WB: Recombinant mouse CYP21B protein; HEK-293T, Jurkat and HL-60 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab232701 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232701于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 55 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 55 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids. -
疾病相关
Adrenal hyperplasia 3 -
序列相似性
Belongs to the cytochrome P450 family. -
结构域
The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1589 Human
- Entrez Gene: 13079 Mouse
- Omim: 613815 Human
- SwissProt: P08686 Human
- SwissProt: P03940 Mouse
- Unigene: 654479 Human
- Unigene: 440407 Mouse
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别名
- CYP21 antibody
- 21 OHase antibody
- 21-OHase antibody
see all
图片
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All lanes : Anti-CYP21A2 antibody (ab232701) at 3 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Anti-CYP21A2 antibody (ab232701) at 3 µg/ml + Recombinant mouse CYP21B protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Paraffin-embedded mouse kidney tissue stained for CYP21A2 using ab232701 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse testis tissue stained for CYP21A2 using ab232701 at 20 µg/ml in immunohistochemical analysis. DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab232701 尚未被引用在任何文献中。