重组Anti-CYP1B1抗体[EPR14972] - C-terminal (ab185954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14972] to CYP1B1 - C-terminal
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CYP1B1抗体[EPR14972] - C-terminal
参阅全部 CYP1B1 一抗 -
描述
兔单克隆抗体[EPR14972] to CYP1B1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human renal adenocarcinoma tissue. WB: Human breast cancer, fetal kidney and fetal brain lysate; Rat brain and spleen lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14972 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185954于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development. -
组织特异性
Expressed in many tissues. -
疾病相关
Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1545 Human
- Entrez Gene: 13078 Mouse
- Entrez Gene: 25426 Rat
- Omim: 601771 Human
- SwissProt: Q16678 Human
- SwissProt: Q64429 Mouse
- SwissProt: Q64678 Rat
- Unigene: 154654 Human
see all -
别名
- Aryl hydrocarbon hydroxylase antibody
- CP1B antibody
- CP1B1_HUMAN antibody
see all
图片
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All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : CYP1B1 knockout A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : SiHa cell lysate
Lane 6 : Human Brain cell lysate
Lane 7 : Human Kidney cell lysate
Lane 8 : Human Liver cell lysate
Lane 9 : HepG2 cell lysate
Lane 10 : LoVo cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Western blot: Anti-CYP1B1 antibody [EPR14972] (ab185954) staining at 1/5000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab185954 was shown to bind specifically to CYP1B1. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in CYP1B1 knockout cell line ab269476 (knockout cell lysate ab269641). To generate this image, wild-type and CYP1B1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 8 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CYP1B1 with ab185954 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (16)
ab185954 被引用在 16 文献中.
- Lidin E et al. Hippocampal Expression of Cytochrome P450 1B1 in Penetrating Traumatic Brain Injury. Int J Mol Sci 23:N/A (2022). PubMed: 35054909
- Wan X et al. Histomorphological and ultrastructural cadmium-induced kidney injuries and precancerous lesions in rats and screening for biomarkers. Biosci Rep 42:N/A (2022). PubMed: 35678542
- Yang L et al. Immutol regulates CD4+Tregs, CD8+Tregs and pDCs via IDO signaling pathway to induce immune tolerance in rat heart allograft transplant. Transpl Immunol 68:101393 (2021). PubMed: 33838298
- Mo Y et al. Bupi Yishen formula attenuates kidney injury in 5/6 nephrectomized rats via the tryptophan-kynurenic acid-aryl hydrocarbon receptor pathway. BMC Complement Med Ther 21:207 (2021). PubMed: 34376166
- Hashimoto Y et al. Suppressor effect of catechol-O-methyltransferase gene in prostate cancer. PLoS One 16:e0253877 (2021). PubMed: 34587154