Application
ELISA
Sample
Human Purified protein (competitive ELISA assay)
Specification
competitive ELISA assay
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Type
Other
Other product details
Dilution
1/300
Incubation time
24 hour(s) and 0 minute(s)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP)
Additional data
Additional Notes
cyclosporine A linked to BSA was coated to 96-well plate (overnight 4C)
wash 3 PBS+Tween and 3X PBS
cyclophilin (0.3 ug/mL. Affinity Bioreagents PAa1-025) + immunosuppressor (10000 to 0.168 ng/mL) was added and incubated overnight (COMPETITION ofr cyclophilin.
rabbit anti cyclophilin A was added overninght 4C
goat anti rabbit 2 hr 37C prior alkaline phosphatase Sigma (P7998)
wash 3 PBS+Tween and 3X PBS
cyclophilin (0.3 ug/mL. Affinity Bioreagents PAa1-025) + immunosuppressor (10000 to 0.168 ng/mL) was added and incubated overnight (COMPETITION ofr cyclophilin.
rabbit anti cyclophilin A was added overninght 4C
goat anti rabbit 2 hr 37C prior alkaline phosphatase Sigma (P7998)
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提交于 Jul 31 2006