重组Anti-Cyclophilin 40抗体[EPR14845(B)] (ab181983)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14845(B)] to Cyclophilin 40
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cyclophilin 40抗体[EPR14845(B)]
参阅全部 Cyclophilin 40 一抗 -
描述
兔单克隆抗体[EPR14845(B)] to Cyclophilin 40 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, JAR, HepG2 and K-562 cell lysate; Human fetal brain lysate; Recombinant Human Cyclophilin 40 protein (BSA and azide free) (ab173064). IHC-P: Human transitional cell carcinoma of bladder. ICC/IF: K562 cell lyaste.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR14845(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181983于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 41 kDa.
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ICC/IF |
1/500.
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说明 |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/500. |
靶标
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功能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5481 Human
- Entrez Gene: 67738 Mouse
- Entrez Gene: 361967 Rat
- Omim: 601753 Human
- SwissProt: Q08752 Human
- SwissProt: Q9CR16 Mouse
- SwissProt: Q6DGG0 Rat
- Unigene: 183958 Human
see all -
别名
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
see all
图片
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All lanes : Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPID knockout HeLa cell lysate
Lane 3 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab181983 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181983 Anti-Cyclophilin 40 antibody [EPR14845(B)] was shown to specifically react with Cyclophilin 40 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265788 (knockout cell lysate ab257600) was used. Wild-type and Cyclophilin 40 knockout samples were subjected to SDS-PAGE. ab181983 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983) at 1/20000 dilution
Lane 1 : Human fetal brain
Lane 2 : JAR
Lane 3 : K562
Lane 4 : HepG2
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa -
Immunofluorescence staining of K562 cells labeling Cyclophilin 40 with ab181983 at 1/200 dilution followed by labeling with Goat
anti rabbit IgG(Alexa Fluor® 555) antibody (1/500 dilution) and counter-staining with Dapi (blue) -
Imunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder labeling Cyclophilin 40 with ab181983 at 1/50 dilution followed by labeing with secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin staining.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab181983 被引用在 3 文献中.
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- Li J et al. Melatonin attenuates renal fibrosis in diabetic mice by activating the AMPK/PGC1a signaling pathway and rescuing mitochondrial function. Mol Med Rep 19:1318-1330 (2019). PubMed: 30535482
- Zhou H et al. Inhibitory effect of melatonin on necroptosis via repressing the Ripk3-PGAM5-CypD-mPTP pathway attenuates cardiac microvascular ischemia-reperfusion injury. J Pineal Res N/A:e12503 (2018). PubMed: 29770487