Anti-Cyclin T2/CCNT2抗体(ab96133)
Key features and details
- Rabbit polyclonal to Cyclin T2/CCNT2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin T2/CCNT2抗体
参阅全部 Cyclin T2/CCNT2 一抗 -
描述
兔多克隆抗体to Cyclin T2/CCNT2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Cyclin T2/CCNT2 aa 497-707.
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阳性对照
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: Hela cell IHC-P: H1299 Xenograft; mouse brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96133于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 81 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 81 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the cyclin family. Cyclin C subfamily. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 905 Human
- Entrez Gene: 72949 Mouse
- Omim: 603862 Human
- SwissProt: O60583 Human
- Unigene: 658324 Human
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别名
- CCNT2 antibody
- CCNT2_HUMAN antibody
- Cyclin T2a antibody
see all
图片
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ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded mouse brain tissue by Immunohistochemistry.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Anti-Cyclin T2/CCNT2 antibody (ab96133) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS PAGE -
ab96133, at a 1/200 dilution, staining Cyclin T2/CCNT2 in paraformaldehyde fixed A431 cells by Immunofluorescence.
Lower image: merged with DNA probe. -
ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded H1299 (human cell line) xenograft by Immunohistochemistry.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab96133 被引用在 2 文献中.
- Xing L et al. Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis. Open Life Sci 15:1013-1023 (2020). PubMed: 33817287
- Oqani RK et al. P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig. PLoS One 11:e0152254 (2016). PubMed: 27011207