Anti-Cyclin T1抗体(ab2098)
Key features and details
- Rabbit polyclonal to Cyclin T1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin T1抗体
参阅全部 Cyclin T1 一抗 -
描述
兔多克隆抗体to Cyclin T1 -
宿主
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
免疫原
Fusion protein (Human).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading...
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纯度
Whole antiserum -
纯化说明
This product was prepared from monospecific antiserum by delipidation and defibrination. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Human
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WB |
Human
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应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).
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IHC-P |
1/200 - 1/1000.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa). |
IHC-P
1/200 - 1/1000. |
靶标
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功能
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the cyclin family. Cyclin C subfamily. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 904 Human
- Omim: 143055 Human
- SwissProt: O60563 Human
- Unigene: 279906 Human
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别名
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
ab2098 diluted 1:500 to detect Cyclin T1 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)ab2098 (2µg/ml) staining Cyclin T1 in human lymph node using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Anti-Cyclin T1 antibody (ab2098) at 1/10000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 48 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
The 90 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Cyclin T1.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab2098 被引用在 14 文献中.
- Bidaux G et al. FRET Image Correlation Spectroscopy Reveals RNAPII-Independent P-TEFb Recruitment on Chromatin. Biophys J 114:522-533 (2018). PubMed: 29414698
- Sakurai N et al. BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene. Sci Rep 7:11962 (2017). PubMed: 28931940
- Lin LF et al. Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors. Mol Clin Oncol 4:971-975 (2016). PubMed: 27284431
- Jin JS et al. Increased cyclin T1 expression as a favorable prognostic factor in treating gastric adenocarcinoma. Oncol Lett 10:3712-3718 (2015). PubMed: 26788195
- Lefèvre L et al. Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of ß-catenin in adrenocortical carcinoma. Oncogenesis 4:e161 (2015). ICC/IF . PubMed: 26214578