Anti-Cyclin T1抗体(ab2098)

Submit a review Q&A (4)References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
  • Western blot - Anti-Cyclin T1 antibody (ab2098)

Key features and details

  • Rabbit polyclonal to Cyclin T1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Cyclin T1抗体
    参阅全部 Cyclin T1 一抗

  • 描述

    兔多克隆抗体to Cyclin T1

  • 宿主

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Fusion protein (Human).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Human
应用 Ab评论 说明
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).
IHC-P
1/200 - 1/1000.
说明
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).
IHC-P
1/200 - 1/1000.

靶标

  • 功能

    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

  • 组织特异性

    Ubiquitously expressed.

  • 序列相似性

    Belongs to the cyclin family. Cyclin C subfamily.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CCN T1 antibody
      • CCNT antibody
      • CCNT 1 antibody
      • Ccnt1 antibody
      • CCNT1_HUMAN antibody
      • CDK9 associated C type protein antibody
      • Cyc T1 antibody
      • Cyclin C related protein antibody
      • Cyclin T antibody
      • cyclin T1 antibody
      • Cyclin T1b antibody
      • Cyclin-T antibody
      • Cyclin-T1 antibody
      • CYCT 1 antibody
      • CycT1 antibody
      • HIVE1 antibody
      • Human immunodeficiency virus 1 expression antibody
      • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
      • pTEFb subunit antibody
      • Subunit of positive elongation transcription factor b antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)

      ab2098 diluted 1:500 to detect Cyclin T1 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab2098)
      ab2098 (2µg/ml) staining Cyclin T1 in human lymph node using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Western blot - Anti-Cyclin T1 antibody (ab2098)
      Western blot - Anti-Cyclin T1 antibody (ab2098)
      Anti-Cyclin T1 antibody (ab2098) at 1/10000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 81 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 48 kDa, 78 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 16 minutes


      The 90 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Cyclin T1.

    文献 (14)

    发表研究结果有使用 ab2098？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2098 被引用在 14 文献中.

    • Bidaux G  et al. FRET Image Correlation Spectroscopy Reveals RNAPII-Independent P-TEFb Recruitment on Chromatin. Biophys J 114:522-533 (2018). PubMed: 29414698
    • Sakurai N  et al. BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene. Sci Rep 7:11962 (2017). PubMed: 28931940
    • Lin LF  et al. Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors. Mol Clin Oncol 4:971-975 (2016). PubMed: 27284431
    • Jin JS  et al. Increased cyclin T1 expression as a favorable prognostic factor in treating gastric adenocarcinoma. Oncol Lett 10:3712-3718 (2015). PubMed: 26788195
    • Lefèvre L  et al. Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of ß-catenin in adrenocortical carcinoma. Oncogenesis 4:e161 (2015). ICC/IF . PubMed: 26214578
    View all Publications for this product

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Question

    BATCH NUMBER 115294 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM more bands, unspecific SAMPLE Cell extract PRIMARY ANTIBODY Abcam, ab2098,Rabbit polyclonal 1:1000, 1h RT, wash 5 times a 5 min with Western wash DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED no, but other batches from you always worked fine ANTIBODY STORAGE CONDITIONS aliquoted a 20ul in -20 SAMPLE PREPARATION Ripa Buffer, Protese Inhibotors AMOUNT OF PROTEIN LOADED 20-50ug ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 10% TRANSFER AND BLOCKING CONDITIONS 1 hour blocking in 5% BSA in Western Wash Buffer SECONDARY ANTIBODY Jackson Immuno Research :Goat anti rabbit IgG-HRP 1:10.000, 1 hr, RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 20 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? nothing altered, since we got fine results with other batch (we are out of this Antidody)

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    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been experiencing definitely appear to be batch related. I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

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    Question

    Customer's enquiry: Is the immunogen sequence available?

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    Answer

    Sorry, the sequence is not available.

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    Question

    Is 85 mg/ml correct?

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    Answer

    The protein concentration is 85 mg/ml.

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    Question

    I am interested in using either cyclin T1-ab2098 or cdk9-ab6544 in a chromatin immunoprecipitation assay using human B cells to identify binding by P-TEFb (PITALRE) in vivo. Have either antibody been used in this assay and which would you recommend trying of the two? Many thanks Dr. Julian knight

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    Answer

    Neither of these antibodies has yet been tested for ChIP, although they work very well in standard IP. We are unable to suggest which of the two may be better. However, we would be very interested to hear the results and will reward you with an amazon.com gift certificate ($30) for you data. Good luck with your experiments,

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