使用敲除细胞株进行验证

Anti-Cyclin E1抗体[HE12] (ab3927)

Reviews (9)Q&A (10)References (44)

Key features and details

  • Mouse monoclonal [HE12] to Cyclin E1
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Cyclin E1抗体[HE12]
    参阅全部 Cyclin E1 一抗

  • 描述

    小鼠单克隆抗体[HE12] to Cyclin E1

  • 宿主

    Mouse

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1.

  • 阳性对照

    • MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells

  • 常规说明

    This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.

性能

应用

  • 应用说明
    Is unsuitable for IHC-Fr or IHC-P.

    • 靶标

    • 功能

      Essential for the control of the cell cycle at the G1/S (start) transition.

    • 组织特异性

      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

    • 序列相似性

      Belongs to the cyclin family. Cyclin E subfamily.

    • 翻译后修饰

      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CCNE antibody
        • Ccne1 antibody
        • CCNE1_HUMAN antibody
        • cyclin E variant ex5del antibody
        • cyclin E variant ex7del antibody
        • Cyclin E1 antibody
        • Cyclin Es antibody
        • Cyclin Et antibody
        • CyclinE antibody
        • G1/S specific cyclin E antibody
        • G1/S-specific cyclin-E1 antibody
        see all

      文献 (44)

      发表研究结果有使用 ab3927？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3927 被引用在 44 文献中.

      • Venugopal N  et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
      • Ju LG  et al. SPOP suppresses prostate cancer through regulation of CYCLIN E1 stability. Cell Death Differ 26:1156-1168 (2019). PubMed: 30237511
      • Zhang L  et al. Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways. Oncol Rep 42:1843-1855 (2019). PubMed: 31432177
      • Li S  et al. Up-regulation of microRNA-200c-3p inhibits invasion and migration of renal cell carcinoma cells via the SOX2-dependent Wnt/ß-catenin signaling pathway. Cancer Cell Int 19:231 (2019). PubMed: 31516388
      • Lee GH  et al. Effects of Fludioxonil on the Cell Growth and Apoptosis in T and B Lymphocytes. Biomolecules 9:N/A (2019). PubMed: 31540454
      View all Publications for this product

      客户评价及客户问答

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      1-10 of 19 Abreviews or Q&A

      Western blot abreview for Anti-Cyclin E1 antibody [HE12]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HepG2 liver cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      50 µg
      Specification
      HepG2 liver cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Apr 22 2015

      Western blot abreview for Anti-Cyclin E1 [HE12] antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (15%)
      Sample
      Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
      Specification
      Mouse Pancreatic cancer
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Feb 25 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin E antibody [HE12]

       Good
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated
      Sample
      Human Tissue sections (endometiral stromal sarcoma)
      Specification
      endometiral stromal sarcoma
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Jul 30 2013

      Question

      many bands in WB
      1) What is the sample type? Metastatic Breast Cancer

      2) What lysis buffer was used? How much protein was loaded? Cell Signaling Lysis Buffer #9803S with protease and phosphatase inhibitors and with 40ug protein loaded.

      3) How long was the primary antibody incubation? overnight for 12 hours

      4) What was the blocking solution and blocking time? Odyssey Blocking Buffer for 1 hour at room temp
      .

      Read More
      Answer

      Thank you for this additional information. One thing to try is using the 1/200 dilution for 1-2 hours room temperature, to see if you can get the target band without getting all the non-specific ones. Please let me know if this does not improve your results.

      Read More

      Question

      I'd be happy to try a replacement aliquot of the cyclin E antibody.

      Read More
      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab3927 with the order number 946601. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Question

      Thanks for you reply. From previous experiments and from the observed phenotype (re-replication) cyclin E should increase in siEmi1_1. What seems strange to me is also the migration, slower than it used to be.

      Read More
      Answer

      Something does seem odd about the western blot data. By itself, the slightly slower migration of the cyclin E band is not that concerning. But together with the apparent insensitivity to cyclin E levels, it appears the new vial of ab3927 you received may not be detecting cyclin E. I would be happy to send you a replacement vial if you are interested.

      Read More

      Question

      Hi, I'm writing concerning the anti-cyclin E monoclonal antibody HE12 (cat ab3927). I have used it in the past and always obtained very good results (which also resulted in a publication, see attached figure, right panel). I am now repeating some of those experiments (left panel on the attached figure) and I've ordered a new vial of the same antibody (order number PR1381431). Surprisingly, I now obtain different results: a) cyclin E seems to migrate slower than in previous experiments (compare left and right panel in the attached figure). It is much closer to the 55kDa molecular marker and slower than the other cyclins. Please note the same pre-cast commercial gels are used in the 2 sets of experiments. b) while cyclin E levels increased in siEmi1 sample in the first set of experiments (right panel), in the most recent experiments there is no difference between control and siRNA sample (while the efficiency of the siRNA is comparable and the other cyclins vary in a similar manner, indicating that the experimental condition are comparable). This was observed in 3 independent experiments. Therefore, I am not sure whether the new batch of antibody is recognising cyclin E. I realise that the two batches of antibody have been ordered few years apart, but since it's a monoclonal I'd have expected them to perform in a similar manner. Do you know if anything has changed in the antibody production? If it's a problem with a specific batch, would it be possible to try an independent one of the same monoclonal?

      Read More
      Answer

      Thank you for your description of the issues and for including the western blot images. So you are expecting cyclin E to levels to increase due to siEmi1_1?

      Read More

      Western blot abreview for Anti-Cyclin E antibody [HE12]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLA cells)
      Loading amount
      20 µg
      Specification
      HeLA cells
      Gel Running Conditions
      Reduced Denaturing (10)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Aug 25 2011

      Western blot abreview for Anti-Cyclin E antibody [HE12]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa cells)
      Loading amount
      20 µg
      Specification
      HeLa cells
      Treatment
      2mM double Thymidine block
      Gel Running Conditions
      Reduced Denaturing (12.5%)
      Blocking step
      Milk as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      提交于 Feb 24 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin E antibody [HE12]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa cells)
      Specification
      HeLa cells
      Fixative
      Methanol
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      提交于 Feb 24 2011

      1-10 of 19 Abreviews or Q&A

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