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Cell Biology Cell Cycle Cyclins
使用敲除细胞株进行验证

Anti-Cyclin E1抗体[HE12] (ab3927)

  • Datasheet
  • SDS
Reviews (9)Q&A (10)References (49)

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Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
  • Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)

Key features and details

  • Mouse monoclonal [HE12] to Cyclin E1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Cyclin E1抗体[HE12]
    参阅全部 Cyclin E1 一抗
  • 描述

    小鼠单克隆抗体[HE12] to Cyclin E1
  • 宿主

    Mouse
  • 经测试应用

    适用于: WBmore details
    不适用于: IHC-Fr or IHC-P
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1.

  • 阳性对照

    • MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
  • 常规说明

    This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    HE12
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin E Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin E Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin E family

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Positive Controls

    • ZR-75-1 nuclear extract lysate (ab14916)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Cyclin E1 protein (ab119719)
  • Unmasking reagent

    • 10x Citrate Buffer pH 6.0 (ab64214)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3927于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (6)
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
  • 应用说明
    Is unsuitable for IHC-Fr or IHC-P.
  • 靶标

    • 功能

      Essential for the control of the cell cycle at the G1/S (start) transition.
    • 组织特异性

      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • 序列相似性

      Belongs to the cyclin family. Cyclin E subfamily.
    • 翻译后修饰

      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession P24864 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 898 Human
      • Omim: 123837 Human
      • SwissProt: P24864 Human
      • Unigene: 244723 Human
      • 别名

        • CCNE antibody
        • Ccne1 antibody
        • CCNE1_HUMAN antibody
        • cyclin E variant ex5del antibody
        • cyclin E variant ex7del antibody
        • Cyclin E1 antibody
        • Cyclin Es antibody
        • Cyclin Et antibody
        • CyclinE antibody
        • G1/S specific cyclin E antibody
        • G1/S-specific cyclin-E1 antibody
        see all

      图片

      • Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
        Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
        All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution

        Lane 1 : Wild-type HAP1 whole cell lysate
        Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Predicted band size: 47 kDa



        Lanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

        ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)
        Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.

        Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (49)

      发表研究结果有使用 ab3927?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3927 被引用在 49 文献中.

      • Venugopal N  et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
      • Mlyczynska E  et al. Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG-3 and BeWo cells proliferation and cell cycle. Int J Mol Med 45:691-702 (2020). PubMed: 31922236
      • Huang R  et al. The activation of GPER inhibits cells proliferation, invasion and EMT of triple-negative breast cancer via CD151/miR-199a-3p bio-axis. Am J Transl Res 12:32-44 (2020). PubMed: 32051735
      • Al-Wabli RI  et al. New Isatin-Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity. Drug Des Devel Ther 14:483-495 (2020). PubMed: 32099332
      • Wang Y  et al. The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer. Cancer Manag Res 11:8081-8093 (2019). PubMed: 31507330
      View all Publications for this product

      客户评价及客户问答

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      提交评价 提交问题

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Cyclin E1 antibody [HE12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HepG2 liver cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      50 µg
      Specification
      HepG2 liver cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Apr 22 2015

      Western blot abreview for Anti-Cyclin E1 [HE12] antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (15%)
      Sample
      Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
      Specification
      Mouse Pancreatic cancer
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Feb 25 2014

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLA cells)
      Loading amount
      20 µg
      Specification
      HeLA cells
      Gel Running Conditions
      Reduced Denaturing (10)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Aug 25 2011

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa cells)
      Loading amount
      20 µg
      Specification
      HeLa cells
      Treatment
      2mM double Thymidine block
      Gel Running Conditions
      Reduced Denaturing (12.5%)
      Blocking step
      Milk as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      提交于 Feb 24 2011

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human Lymphocytes)
      Loading amount
      1.5e+006 cells
      Specification
      Human Lymphocytes
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      提交于 Apr 07 2008

      Western blot abreview for Anti-Cyclin E antibody [HE12]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (human ovarian cancer cell lines)
      Specification
      human ovarian cancer cell lines
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
      Read More

      Abcam user community

      Verified customer

      提交于 Jun 22 2006

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