Key features and details
- Mouse monoclonal [HE12] to Cyclin E1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
参阅全部 Cyclin E1 一抗
描述小鼠单克隆抗体[HE12] to Cyclin E1
经测试应用适用于: WBmore details
不适用于: IHC-Fr or IHC-P
Synthetic peptide corresponding to Human Cyclin E1.
- MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab3927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.|
功能Essential for the control of the cell cycle at the G1/S (start) transition.
组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
序列相似性Belongs to the cyclin family. Cyclin E subfamily.
翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDa
Lanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.
ab3927 被引用在 47 文献中.
- Venugopal N et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
- Mlyczynska E et al. Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG-3 and BeWo cells proliferation and cell cycle. Int J Mol Med 45:691-702 (2020). PubMed: 31922236
- Huang R et al. The activation of GPER inhibits cells proliferation, invasion and EMT of triple-negative breast cancer via CD151/miR-199a-3p bio-axis. Am J Transl Res 12:32-44 (2020). PubMed: 32051735
- Al-Wabli RI et al. New Isatin-Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity. Drug Des Devel Ther 14:483-495 (2020). PubMed: 32099332
- Ju LG et al. SPOP suppresses prostate cancer through regulation of CYCLIN E1 stability. Cell Death Differ 26:1156-1168 (2019). PubMed: 30237511