Anti-Cyclin E1抗体[HE12] (ab3927)
![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.cn/ps/products/3/ab3927/Images/ab3927-319310-anti-cyclin-e1-antibody-he12-western-blot.jpg "Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)")
Key features and details
- Mouse monoclonal [HE12] to Cyclin E1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Cyclin E1抗体[HE12]
参阅全部 Cyclin E1 一抗 -
描述
小鼠单克隆抗体[HE12] to Cyclin E1 -
宿主
Mouse -
经测试应用
适用于: WBmore details
不适用于: IHC-Fr or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Cyclin E1.
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阳性对照
- MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
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常规说明
This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
HE12 -
骨髓瘤
unknown -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Unmasking reagent
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. |
靶标
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功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
组织特异性
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
序列相似性
Belongs to the cyclin family. Cyclin E subfamily. -
翻译后修饰
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 898 Human
- Omim: 123837 Human
- SwissProt: P24864 Human
- Unigene: 244723 Human
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别名
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
图片
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Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDaLanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.cn/ps/products/3/ab3927/Images/ab3927-8419-anti-cyclin-e1-antibody-he12-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (49)
ab3927 被引用在 49 文献中.
- Venugopal N et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
- Mlyczynska E et al. Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG-3 and BeWo cells proliferation and cell cycle. Int J Mol Med 45:691-702 (2020). PubMed: 31922236
- Huang R et al. The activation of GPER inhibits cells proliferation, invasion and EMT of triple-negative breast cancer via CD151/miR-199a-3p bio-axis. Am J Transl Res 12:32-44 (2020). PubMed: 32051735
- Al-Wabli RI et al. New Isatin-Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity. Drug Des Devel Ther 14:483-495 (2020). PubMed: 32099332
- Wang Y et al. The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer. Cancer Manag Res 11:8081-8093 (2019). PubMed: 31507330