Anti-Cyclin E1抗体[HE12] (ab3927)
![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.cn/ps/products/3/ab3927/Images/ab3927-319310-anti-cyclin-e1-antibody-he12-western-blot.jpg "Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)")
Key features and details
- Mouse monoclonal [HE12] to Cyclin E1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Cyclin E1抗体[HE12]
参阅全部 Cyclin E1 一抗 -
描述
小鼠单克隆抗体[HE12] to Cyclin E1 -
宿主
Mouse -
经测试应用
适用于: WBmore details
不适用于: IHC-Fr or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Cyclin E1.
-
阳性对照
- MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. NIH3T3 cells
-
常规说明
This antibody may be diluted to a titer of 1:100 - 1:200 with an enzyme-polymer detection method. The Final dilution sould be determined by the user based upon the staining conditions employed.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
HE12 -
骨髓瘤
unknown -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Unmasking reagent
应用
Our Abpromise guarantee covers the use of ab3927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. |
靶标
-
功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
组织特异性
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
序列相似性
Belongs to the cyclin family. Cyclin E subfamily. -
翻译后修饰
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 898 Human
- Omim: 123837 Human
- SwissProt: P24864 Human
- Unigene: 244723 Human
-
别名
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
图片
-
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)All lanes : Anti-Cyclin E1 antibody [HE12] (ab3927) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CCNE1 (Cyclin E1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDaLanes 1 - 2: Merged signal (red and green). Green - ab3927 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab3927 was shown to recognize CCNE1 in wild-type HAP1 cells as signal was lost at the expected MW in CCNE1 (Cyclin E1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CCNE1 (Cyclin E1) knockout samples were subjected to SDS-PAGE. Ab3927 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)](https://www.abcam.cn/ps/products/3/ab3927/Images/ab3927-8419-anti-cyclin-e1-antibody-he12-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [HE12] (ab3927)Image kindly provided by Tulsiram Prathapam, as part of an abreview.Immunoblot analysis of Cyclin E expression in NIH/3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysed by immunoblotting with ab3927. Immunoblot analysis of Cyclin E expression in NIH3T3 cells. Cells were transfected with the indicated plasmids and harvested 48 hr post transfection. Cyclin E expression was analysised by immunoblotting with ab3927.
实验方案
数据表及文件
文献 (47)
ab3927 被引用在 47 文献中.
- Venugopal N et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
- Mlyczynska E et al. Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG-3 and BeWo cells proliferation and cell cycle. Int J Mol Med 45:691-702 (2020). PubMed: 31922236
- Huang R et al. The activation of GPER inhibits cells proliferation, invasion and EMT of triple-negative breast cancer via CD151/miR-199a-3p bio-axis. Am J Transl Res 12:32-44 (2020). PubMed: 32051735
- Al-Wabli RI et al. New Isatin-Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity. Drug Des Devel Ther 14:483-495 (2020). PubMed: 32099332
- Ju LG et al. SPOP suppresses prostate cancer through regulation of CYCLIN E1 stability. Cell Death Differ 26:1156-1168 (2019). PubMed: 30237511
