重组Anti-Cyclin D3/CCND3抗体[1/Cyclin D3] - BSA and Azide free (ab289556)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1/Cyclin D3] to Cyclin D3/CCND3 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Cyclin D3/CCND3抗体[1/Cyclin D3] - BSA and Azide free
参阅全部 Cyclin D3/CCND3 一抗 -
描述
小鼠单克隆抗体[1/Cyclin D3] to Cyclin D3/CCND3 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: K562, Jurkat, HeLa, Daudi, HEL, PC-12, A20, C6 and C2C12 whole cell lysate. Human and mouse lymphoma lysates. IHC-P: Mouse and rat spleen and stomach tissue. IP: C2C12 cell lysate.
-
常规说明
ab289556 is the carrier-free version of ab289546
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1/Cyclin D3 -
同种型
IgG2b -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab289556于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
序列相似性
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
细胞定位
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. - Information by UniProt
-
数据库链接
- Entrez Gene: 896 Human
- Entrez Gene: 12445 Mouse
- Entrez Gene: 25193 Rat
- Omim: 123834 Human
- SwissProt: P30281 Human
- SwissProt: P30282 Mouse
- SwissProt: P48961 Rat
- Unigene: 534307 Human
see all -
别名
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
see all
图片
-
All lanes : Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (ab289546) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 5 : HEL (erythroleukemia erythroblast), whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 7 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 8 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 9 : C2C12 (mouse myoblast), whole cell lysate
Lane 10 : Human lymphoma lysate
Lane 11 : Mouse lymphoma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDaThis data was developed using ab289546, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-7/9-11: 37 seconds ; Lane 8: 3 minutes.
-
This data was developed using ab289546 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining in mouse spleen. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Sample was counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab289546 the same antibody clone in a different buffer formulation.
Cyclin D3/CCND3 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) whole cell lysate 10 μg with ab289546 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289546 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: C2C12 whole cell lysate 10 μg
Lane 2: ab289546 IP in C2C12 whole cell lysate
Lane 3: Mouse IgG2b instead of ab289546 in C2C12 whole cell lysate
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 32 seconds.
-
This data was developed using ab289546 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Sporadically nuclear staining in mouse stomach. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The sample was cunterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab289546 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining in rat spleen. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The sample was counterstained with Hematoxylin.
Secondary antibody only control: secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab289546 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Sporadically nuclear staining in rat stomach. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The sample was counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab289556 尚未被引用在任何文献中。