Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCAR cell line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100 µg
Treatment
Perifosine 0/12/24h
Specification
Epithelial OVCAR cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Detection
Detection method
ECL Western Blotting Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 21 2016