Anti-Cyclin B2/CCNB2抗体[X121.10] (ab10839)
Key features and details
- Mouse monoclonal [X121.10] to Cyclin B2/CCNB2
- Suitable for: WB
- Reacts with: Xenopus laevis
- Isotype: IgG1
概述
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产品名称
Anti-Cyclin B2/CCNB2抗体[X121.10]
参阅全部 Cyclin B2/CCNB2 一抗 -
描述
小鼠单克隆抗体[X121.10] to Cyclin B2/CCNB2 -
宿主
Mouse -
特异性
ab10839 blots only cyclin B2/CCNB2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Xenopus laevis -
免疫原
Full length native protein (purified) corresponding to Xenopus laevis Cyclin B2/CCNB2.
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表位
The epitope for X121 lies within a region spanning the amino acids 1-112. -
阳性对照
- Xenopus egg extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
X121.10 -
骨髓瘤
Sp2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10839于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. |
靶标
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功能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
序列相似性
Belongs to the cyclin family. Cyclin AB subfamily. -
发展阶段
Accumulates steadily during G2 and is abruptly destroyed at mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 397743 Xenopus laevis
- SwissProt: P13351 Xenopus laevis
- Unigene: 34562 Xenopus laevis
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别名
- ccnb2 antibody
- CCNB2_HUMAN antibody
- CycB2 antibody
see all
图片
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All lanes : Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) at 1 µg/ml
Lane 1 : Interphase Xenopus egg extracts (cyclin B destroyed)
Lane 2 : Mitotic (CSF) Xenopus egg extracts (cyclin B present)
Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa (possible cross reactivity), 40 kDa (possible cross reactivity)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab10839 被引用在 5 文献中.
- Li H et al. Integrative module analysis of HCC gene expression landscapes. Exp Ther Med 19:1779-1788 (2020). PubMed: 32104233
- Lin R et al. Electroacupuncture ameliorate learning and memory by improving N-acetylaspartate and glutamate metabolism in APP/PS1 mice. Biol Res 51:21 (2018). PubMed: 29980225
- Hansen DV et al. Emi2 at the crossroads: where CSF meets MPF. Cell Cycle 6:732-8 (2007). PubMed: 17361104
- Moore JD et al. Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. Mol Biol Cell 13:4388-400 (2002). PubMed: 12475960
- Bodart JF et al. Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis. Exp Cell Res 253:413-21 (1999). PubMed: 10585264