Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
产品名称Anti-Cyclin B1 (phospho S126)抗体
参阅全部 Cyclin B1 一抗
描述兔多克隆抗体to Cyclin B1 (phospho S126)
特异性Minimal reactivity is expected with the non-phosphorylated form of the protein.
经测试应用适用于: WBmore details
预测可用于: Mouse, Rat, Hamster, Dog, Chimpanzee
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Our Abpromise guarantee covers the use of ab3488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
功能Essential for the control of the cell cycle at the G2/M (mitosis) transition.
序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
翻译后修饰Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : H23 whole cell lysate
Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
Lane 4 : Glioblastoma (T98G) cell lysate
Lane 5 : B cell lymphoblast (Daudi) cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 20 seconds
ab3488 被引用在 1 文献中.
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297