概述

  • 产品名称

    Anti-Cyclin B1抗体[V152] (HRP)
    参阅全部 Cyclin B1 一抗
  • 描述

    小鼠单克隆抗体[V152] to Cyclin B1 (HRP)
  • 宿主

    Mouse
  • 偶联物

    HRP
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Fusion protein corresponding to Hamster Cyclin B1.

  • 阳性对照

    • In Western Blot, this antibody gave a positive signal in the following whole cell lysates: K562; Jurkat; HEK293. IHC-P: FFPE human tonsil (normal) tissue sections.
  • 常规说明

     

     

性能

应用

Our Abpromise guarantee covers the use of ab201853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

Abcam recommends using 5% Milk as the blocking agent.

靶标

  • 功能

    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 序列相似性

    Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻译后修饰

    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接

  • 别名

    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

图片

  • All lanes : Anti-Cyclin B1 antibody [V152] (HRP) (ab201853) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CCNB1 (Cyclin B1) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 48 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    ab201853 was shown to specifically react with Cyclin B1 in wild-type HAP1 cells as signal was lost in CCNB1 (Cyclin B1) knockout cells. Wild-type and CCNB1 (Cyclin B1) knockout samples were subjected to SDS-PAGE. Ab201853 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • IHC image of Cyclin B1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201853, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Cyclin B1 antibody [V152] (HRP) (ab201853) at 1/5000 dilution

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 51 kDa why is the actual band size different from the predicted?
    Additional bands at: 36 kDa (possible non-specific binding)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201853 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

文献

ab201853 has not yet been referenced specifically in any publications.

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