Anti-Cyclin B1抗体[V152] (ab72)
![Western blot - Anti-Cyclin B1 antibody [V152] (ab72)](https://www.abcam.cn/ps/products/0/ab72/Images/ab72-277468-anti-cyclin-b1-antibody-v152-western-blot.jpg "Western blot - Anti-Cyclin B1 antibody [V152] (ab72)")
Key features and details
- Mouse monoclonal [V152] to Cyclin B1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Cyclin B1抗体[V152]
参阅全部 Cyclin B1 一抗 -
描述
小鼠单克隆抗体[V152] to Cyclin B1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Fusion protein within Hamster Cyclin B1. The exact sequence is proprietary.
Database link: P14635 -
阳性对照
- WB: HeLa, Daudi, K562, Jurkat and HEK-293 whole cell lysate. IHC-P: Human tonsil tissue. Flow Cytometry: HeLa cells.
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常规说明
This antibody clone is manufactured by Abcam.
This monoclonal antibody to cyclin B1 has been knockout validated in Western blot. The expected band for cyclin B1 was observed in wild type cells and the band was not seen in knockout cells.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
V152 -
骨髓瘤
Sp2 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab72 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). Abcam recommends using 5% Milk as the blocking agent. |
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| IHC-P | Use a concentration of 5 µg/ml. |
靶标
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功能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
序列相似性
Belongs to the cyclin family. Cyclin AB subfamily. -
发展阶段
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
翻译后修饰
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- Unigene: 23960 Human
- Unigene: 260114 Mouse
- Unigene: 380027 Mouse
see all -
别名
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
图片
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Western blot - Anti-Cyclin B1 antibody [V152] (ab72)
Lanes:
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Cyclin B1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab72 observed at 55 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab72 detected the expected band for Cyclin B1 in wild type HAP1 cells and the band was not seen in Cyclin B1 knockout HAP1 cells. Wild-type and Cyclin B1 knockout samples were subjected to SDS-PAGE. Ab72 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] (ab72)](https://www.abcam.cn/ps/products/0/ab72/Images/ab72-3288-anti-cyclin-b1-antibody-v152-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] (ab72)ab72 staining Cyclin B1 in human tonsil tissue - formalin-fixed paraffin-embedded section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab72, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-Cyclin B1 antibody [V152] (ab72)](https://www.abcam.cn/ps/products/0/ab72/Images/ab72-3289-anti-cyclin-b1-antibody-v152-western-blot.jpg)
Western blot - Anti-Cyclin B1 antibody [V152] (ab72)All lanes : Anti-Cyclin B1 antibody [V152] (ab72) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 :Daudi whole cell lysate (ab3951)
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate
Lane 5 : HEK-293 (Human embryonic kidney cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent. -
![Western blot - Anti-Cyclin B1 antibody [V152] (ab72)](https://www.abcam.cn/ps/products/0/ab72/Images/ab72-3287-anti-cyclin-b1-antibody-v152-western-blot.jpg)
Western blot - Anti-Cyclin B1 antibody [V152] (ab72)Lanes 1-9 shows the Cyclin B1 wave as HeLa cells enter and exit mitosis. HeLa cells were synchronised in the early S phase by double thymidine block then released to synchronously enter mitosis. Time points were taken at the points indicated.
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate loaded at 25 µg per lane. ab72 used at 1/500 in reducing conditions in conjunction with goat anti mouse (HRP) 1/20,000.
This image is courtesy of an Abreview submitted on 12 September 2005. We do not have any further information relating to this image.
数据表及文件
文献 (79)
ab72 被引用在 79 文献中.
- Luong XG et al. Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption. Nucleic Acids Res 48:3257-3276 (2020). PubMed: 31970406
- Yao J et al. circGFRA1 Enhances NSCLC Progression by Sponging miR-188-3p. Onco Targets Ther 13:549-558 (2020). PubMed: 32021297
- Levasseur MD et al. Aneuploidy in Oocytes Is Prevented by Sustained CDK1 Activity through Degron Masking in Cyclin B1. Dev Cell 48:672-684.e5 (2019). PubMed: 30745144
- Daldello EM et al. Cyclin B2 is required for progression through meiosis in mouse oocytes. Development 146:N/A (2019). PubMed: 30952665
- Rodríguez C et al. A novel human Cdh1 mutation impairs anaphase promoting complex/cyclosome activity resulting in microcephaly, psychomotor retardation, and epilepsy. J Neurochem 151:103-115 (2019). PubMed: 31318984
