- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称Anti-Cyclin A2抗体[E67.1]
参阅全部 Cyclin A2 一抗 -
描述小鼠单克隆抗体[E67.1] to Cyclin A2
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宿主Mouse
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经测试应用适用于: ICC/IF, WB, ICC, IP, IHC-Pmore details
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种属反应性与反应: Mouse, Cow, Human, African green monkey
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免疫原
Full length protein (Cow).
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表位The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1
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常规说明Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号E67.1
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骨髓瘤unknown
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同种型IgG2a
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab39 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 18077453 | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. | |
| ICC | Use at an assay dependent concentration. | |
| IP | Use a concentration of 2.5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
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序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
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发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
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细胞定位Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
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数据库链接
- Entrez Gene: 281667 Cow
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Omim: 123835 Human
- SwissProt: P30274 Cow
- SwissProt: P20248 Human
- SwissProt: P51943 Mouse
- Unigene: 58974 Human
see all -
别名
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
图片
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![Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (ab39)](https://www.abcam.cn/ps/products/0/ab39/Images/ab39_1.jpg)
Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (ab39)Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E67.1] (ab39)](https://www.abcam.cn/ps/products/0/ab39/Images/Cyclin-A-Primary-antibodies-ab39-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E67.1] (ab39)Image courtesy of an anonymous Abreview.ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.
实验方案
文献
This product has been referenced in:
- Sahara N et al. Characteristics of TBS-extractable hyperphosphorylated tau species: aggregation intermediates in rTg4510 mouse brain. J Alzheimers Dis 33:249-63 (2013). Read more (PubMed: 22941973) »
- Gao Z & Godbout R Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin. Cell Signal 23:555-65 (2011). WB . Read more (PubMed: 21111810) »
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