Key features and details
- Mouse monoclonal [E67.1] to Cyclin A2
- Suitable for: IP
- Reacts with: Mouse, Cow, Human
- Isotype: IgG2a
参阅全部 Cyclin A2 一抗
描述小鼠单克隆抗体[E67.1] to Cyclin A2
经测试应用适用于: IPmore details
种属反应性与反应: Mouse, Cow, Human
Full length protein (Cow).
表位The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1
常规说明Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab39 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2.5 µg/ml.|
功能Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
细胞定位Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
ab39 被引用在 4 文献中.
- Sahara N et al. Characteristics of TBS-extractable hyperphosphorylated tau species: aggregation intermediates in rTg4510 mouse brain. J Alzheimers Dis 33:249-63 (2013). PubMed: 22941973
- Gao Z & Godbout R Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin. Cell Signal 23:555-65 (2011). WB . PubMed: 21111810
- Ge XQ & Blow JJ Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories. J Cell Biol 191:1285-97 (2010). IP ; Human . PubMed: 21173116
- Ho KK et al. Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network. J Biol Chem 283:5477-85 (2008). ICC/IF ; Mouse . PubMed: 18077453