Anti-Cyclin A2抗体[E67.1] (ab39)

Reviews (4)Q&A (12)References (7)
Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (ab39)

    Key features and details

    • Mouse monoclonal [E67.1] to Cyclin A2
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG2a

    概述

    • 产品名称

      Anti-Cyclin A2抗体[E67.1]
      参阅全部 Cyclin A2 一抗

    • 描述

      小鼠单克隆抗体[E67.1] to Cyclin A2

    • 宿主

      Mouse

    • 经测试应用

      适用于: IPmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length protein corresponding to Cow Cyclin A2.

    • 表位

      The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1

    • 常规说明

      Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab39于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IP (1)
    Use a concentration of 2.5 µg/ml.
    说明
    IP
    Use a concentration of 2.5 µg/ml.

    靶标

    • 功能

      Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.

    • 序列相似性

      Belongs to the cyclin family. Cyclin AB subfamily.

    • 发展阶段

      Accumulates steadily during G2 and is abruptly destroyed at mitosis.

    • 细胞定位

      Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CCN1 antibody
        • CCNA antibody
        • Ccna2 antibody
        • CCNA2_HUMAN antibody
        • Cyclin A2 antibody
        • Cyclin-A antibody
        • Cyclin-A2 antibody
        see all

      图片

      • Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (ab39)
        Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (ab39)
        Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.

      文献 (7)

      发表研究结果有使用 ab39？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab39 被引用在 7 文献中.

      • Velásquez ZD  et al. Besnoitia besnoiti-driven endothelial host cell cycle alteration. Parasitol Res 119:2563-2577 (2020). PubMed: 32548739
      • Manshouri R  et al. ZEB1/NuRD complex suppresses TBC1D2b to stimulate E-cadherin internalization and promote metastasis in lung cancer. Nat Commun 10:5125 (2019). PubMed: 31719531
      • Ðuraševic S  et al. The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats. Int J Mol Sci 20:N/A (2019). PubMed: 31731785
      • Sahara N  et al. Characteristics of TBS-extractable hyperphosphorylated tau species: aggregation intermediates in rTg4510 mouse brain. J Alzheimers Dis 33:249-63 (2013). PubMed: 22941973
      • Gao Z & Godbout R Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin. Cell Signal 23:555-65 (2011). WB . PubMed: 21111810
      • Ge XQ & Blow JJ Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories. J Cell Biol 191:1285-97 (2010). IP ; Human . PubMed: 21173116
      • Ho KK  et al. Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network. J Biol Chem 283:5477-85 (2008). ICC/IF ; Mouse . PubMed: 18077453

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      Immunoprecipitation abreview for Anti-Cyclin A antibody [E67.1]

       Excellent
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      African Green Monkey Cell lysate - whole cell (CV-1)
      Total protein in input
      500 µg
      Specification
      CV-1
      Immuno-precipitation step
      Protein A/G
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      提交于 May 21 2012

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