Key features and details
- Rabbit polyclonal to Cyclin A2
- Suitable for: WB, IP, ELISA, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
参阅全部 Cyclin A2 一抗
描述兔多克隆抗体to Cyclin A2
特异性Antiserum will specifically react with a 60 kDa Cyclin A2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin A from other species may also occur.
经测试应用适用于: WB, IP, ELISA, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Rabbit, Human
Synthetic peptide corresponding to Human Cyclin A2.
Database link: P20248
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.01% Sodium azide
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纯化说明This product was prepared from monospecific antiserum by delipidation and defibrination.
Our Abpromise guarantee covers the use of ab2097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 49 kDa.|
|ELISA||1/5000 - 1/20000.|
|ICC/IF||1/50. PubMed: 18083977|
功能Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
细胞定位Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- Information by UniProt
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
ab2097, staining Cyclin A in rabbit vascular smooth muscle cells, by Immunocytochemistry/ Immunofluorescence.
Cells were either untreated (left) or treated with 10 µM ceramide (right), fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated in blocking buffer (PBS, 2% skim milk, and 0.1% Tween-20) for 30 min at room temperature. Samples were incubated with primary antibody at 1/50 dilution for 1 hour at room temperature. An AlexaFluor®-conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.
ab2097 被引用在 4 文献中.
- Yuan F & Wang W MicroRNA-802 suppresses breast cancer proliferation through downregulation of FoxM1. Mol Med Rep 12:4647-4651 (2015). PubMed: 26080894
- Touati SA et al. Cyclin A2 is required for sister chromatid segregation, but not separase control, in mouse oocyte meiosis. Cell Rep 2:1077-87 (2012). PubMed: 23122964
- Faustino RS et al. Ceramide regulation of nuclear protein import. J Lipid Res 49:654-62 (2008). ICC/IF ; Rabbit . PubMed: 18083977
- Rao HV et al. Cyclin D1, cdk4, and Bim are involved in thrombin-induced apoptosis in cultured cortical neurons. J Neurochem 101:498-505 (2007). WB ; Rat . PubMed: 17254021