Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10% Invitrogen gel)
Loading amount
40 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA/PBST
Dilution
1/250
Secondary antibody
Dilution
1/5000
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
Detection
Detection method
Odyssey IR
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 41 kDa
Positive control
Lysed WT C57BL/6 mouse heart samples
Additional data
Additional Notes
Images show an alpha-tubulin loading control (green). I have grey scale of just the CXCR4 bands or red only channel if you want it.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Dan Bromage
Verified customer
提交于 Dec 30 2015