Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 min
Permeabilization
No
Specification
colon
Blocking step
3% H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako Antibody Diluent
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Leica refine anti-mouse, anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
various antigen regimens tested, EDTA gave best signal to background
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 27 2014