Key features and details
- Goat polyclonal to CXCR4
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab1670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
功能Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
组织特异性Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
疾病相关Defects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
序列相似性Belongs to the G-protein coupled receptor 1 family.
结构域The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
翻译后修饰Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
细胞定位Cell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
- Information by UniProt
- C-X-C chemokine receptor type 4 antibody
- CD184 antibody
- CD184 antigen antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling CXCR4 with ab1670.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fetal brain tissue labeling CXCR4 with ab1670 at 4 µg/ml. Tissue was fixed in formaldehyde; 1%BSA was used to block tissue for 30 minutes at 25°C. Heat mediated antigen retrieval was performed using a citrate buffer. A polyclonal rabbbit anti-goat IgG Biotin conjugated secondary antibody was used at 1/500 dilution. A good staining of neural cells was observed.
ab1670 staining CXCR4 in Mouse Asthma (OVA) lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in PFA and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0). The primary antibody was diluted 1/100 in 1% PBS-BSA and incubated with the sample for 16 hours at 4°C. A Biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500. CXCR4 is revealed in blue (Histogreen) and nuclei are counterstained in red (Neutral Red)
ab1670 被引用在 44 文献中.
- Wu Y et al. LncNORAD interference inhibits tumor growth and lung cancer cell proliferation, invasion and migration by down-regulating CXCR4 to suppress RhoA/ROCK signaling pathway. Eur Rev Med Pharmacol Sci 24:5446-5455 (2020). PubMed: 32495879
- Zheng Y et al. Establishment of cell lines with porcine spermatogonial stem cell properties. J Anim Sci Biotechnol 11:33 (2020). PubMed: 32308978
- Xie F et al. TUG1 Promoted Tumor Progression by Sponging miR-335-5p and Regulating CXCR4-Mediated Infiltration of Pro-Tumor Immunocytes in CTNNB1-Mutated Hepatoblastoma. Onco Targets Ther 13:3105-3115 (2020). PubMed: 32341656
- Plaza Reyes A et al. Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells. Nat Commun 11:1609 (2020). PubMed: 32231223
- Miao G et al. TLR2/CXCR4 coassociation facilitates Chlamydia pneumoniae infection-induced atherosclerosis. Am J Physiol Heart Circ Physiol 318:H1420-H1435 (2020). PubMed: 32330088