重组Anti-HSPB7抗体[EPR10106(B)] (ab150390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10106(B)] to HSPB7
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HSPB7抗体[EPR10106(B)]
参阅全部 HSPB7 一抗 -
描述
兔单克隆抗体[EPR10106(B)] to HSPB7 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human HSPB7 aa 1-100. The exact sequence is proprietary.
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阳性对照
- Human fetal heart cell lysate; Human skeletal muscle and heart tissues, mouse and rat heart tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.14% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10106(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150390于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
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IP | (1) |
1/10 - 1/100.
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说明 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 19 kDa. |
IP
1/10 - 1/100. |
靶标
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组织特异性
Isoform 1 is highly expressed in adult and fetal heart, skeletal muscle, and at a much lower levels in adipose tissue and in aorta. Undetectable in other tissues. Isoform 2 and isoform 3 are poorly detected in heart. -
序列相似性
Belongs to the small heat shock protein (HSP20) family. -
细胞定位
Cytoplasm. Nucleus. Nucleus > Cajal body. Resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 27129 Human
- Entrez Gene: 29818 Mouse
- Entrez Gene: 50565 Rat
- Omim: 610692 Human
- SwissProt: Q9UBY9 Human
- SwissProt: P35385 Mouse
- SwissProt: Q9QUK5 Rat
- Unigene: 502612 Human
see all -
别名
- Cardiovascular heat shock protein antibody
- cvHsp antibody
- DKFZp779D0968 antibody
see all
图片
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All lanes : Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysates
Lane 2 : Rat heart tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 19 kDa
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysates
Lane 2 : Human skeletal muscle tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 19 kDa
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/200 dilution
All lanes : Mouse embryonic heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit polyclonal HRP conjugated antibody at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 2 minutesBocking carried out at 4oC for 12 hours with 2.5% BSA in TBST. Dilutions carried out in Blocking buffer.
Gel used 4-12%
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Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution + Human fetal heart cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa -
ab150390 immunoprecipitating HSPB7. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: Mouse heart whole cell lysate (10ug)
Lane 2: Mouse heart whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab150390 in mouse heart whole cell lysate -
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling HSPB7 with ab150390 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab150390 staining HSPB7 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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Immunohistochemical analysis of paraffin embedded Human Fetal heart tissue using ab150390 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human heart tissue labeling HSPB7 with ab150390 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab150390 staining HSPB7 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150390 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150390 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150390 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab150390 被引用在 11 文献中.
- Qin W et al. Chronic exposure to ammonia induces oxidative stress and enhanced glycolysis in lung of piglets. Environ Toxicol 37:179-191 (2022). PubMed: 34806272
- Schuldt M et al. Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy. Front Cardiovasc Med 8:612215 (2021). PubMed: 33732734
- Sun X et al. Effect of heat-shock protein B7 on oxidative stress in adipocytes from preruminant calves. J Dairy Sci 102:5673-5685 (2019). PubMed: 30954260
- Dorsch LM et al. Protein Quality Control Activation and Microtubule Remodeling in Hypertrophic Cardiomyopathy. Cells 8:N/A (2019). PubMed: 31323898
- Zhang C et al. Hsd3b2 associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells. J Cell Biochem 119:1111-1121 (2018). PubMed: 28703914