重组Anti-Cullin 1/CUL-1抗体[EPR3103Y] (ab75817)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3103Y] to Cullin 1/CUL-1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cullin 1/CUL-1抗体[EPR3103Y]
参阅全部 Cullin 1/CUL-1 一抗 -
描述
兔单克隆抗体[EPR3103Y] to Cullin 1/CUL-1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Cullin 1/CUL-1 aa 750 to the C-terminus (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa, MCF7, A549 and PC12 cell lysates; human cervical carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3103Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75817于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/20.
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
For unpurified, use 1/2500 - 1/5000. |
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IP |
1/10 - 1/50.
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IHC-P |
1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/20. |
ICC/IF
1/100 - 1/250. |
WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). For unpurified, use 1/2500 - 1/5000. |
IP
1/10 - 1/50. |
IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. -
组织特异性
Expressed in lung fibroblasts. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the cullin family. -
翻译后修饰
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
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数据库链接
- Entrez Gene: 8454 Human
- Entrez Gene: 26965 Mouse
- Entrez Gene: 362356 Rat
- Omim: 603134 Human
- SwissProt: Q13616 Human
- SwissProt: Q9WTX6 Mouse
- Unigene: 146806 Human
- Unigene: 87611 Mouse
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别名
- CUL 1 antibody
- CUL-1 antibody
- CUL1 antibody
see all
图片
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All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817) at 1/1000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MDA-MB-435 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab75817 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of HeLa cells with purified ab75817 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75817 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cullin 1/CUL-1 with purified ab75817 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817) at 1/1000 dilution (purified)
Lane 1 : PC-12 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : mouse brain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab75817 (purified) at 1/120 immunoprecipitating Cullin 1 in 10 μg MCF7 whole cell lysate (Lanes 1 and 2, observed at 90 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab75817 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Unpurified ab75817 at 1/100 dilution staining Cullin 1/CUL-1 in human cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3103Y] (ab75817) at 1/5000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
Unpurified ab75817 showing positive staining in Normal human tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab75817 showing positive staining in human Urinary bladder transitional carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab75817 showing positive staining in human Ovarian carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab75817 被引用在 25 文献中.
- Gupta S et al. Functional Characterization of Non-Ankyrin Repeat Domains of Orientia tsutsugamushi Ank Effectors Reveals Their Importance for Molecular Pathogenesis. Infect Immun 90:e0062821 (2022). PubMed: 35435726
- Xia S et al. TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance. Cell Mol Gastroenterol Hepatol 12:1121-1143 (2021). PubMed: 33962073
- Adcox HE et al. Orientia tsutsugamushi Nucleomodulin Ank13 Exploits the RaDAR Nuclear Import Pathway To Modulate Host Cell Transcription. mBio 12:e0181621 (2021). PubMed: 34340535
- Dong X et al. Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma. Aging (Albany NY) 13:10603-10618 (2021). PubMed: 33833131
- Du MG et al. The Absence of PTEN in Breast Cancer Is a Driver of MLN4924 Resistance. Front Cell Dev Biol 9:667435 (2021). PubMed: 33996822