重组Anti-Cubilin抗体[EPR16259-34] - C-terminal (ab191074)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16259-34] to Cubilin - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cubilin抗体[EPR16259-34] - C-terminal
参阅全部 Cubilin 一抗 -
描述
兔单克隆抗体[EPR16259-34] to Cubilin - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal kidney lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16259-34 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/40.
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WB |
1/1000 - 1/2000. Detects a band of approximately 399 kDa (predicted molecular weight: 399 kDa).
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说明 |
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IP
1/40. |
WB
1/1000 - 1/2000. Detects a band of approximately 399 kDa (predicted molecular weight: 399 kDa). |
靶标
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功能
Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. -
组织特异性
Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney. -
疾病相关
Recessive hereditary megaloblastic anemia 1 -
序列相似性
Contains 27 CUB domains.
Contains 7 EGF-like domains. -
结构域
The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2. -
翻译后修饰
The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off.
N-glycosylated. -
细胞定位
Endosome membrane. Lysosome membrane. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 8029 Human
- Omim: 602997 Human
- SwissProt: O60494 Human
- Unigene: 166206 Human
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别名
- 460 kDa receptor antibody
- cubilin (intrinsic factor-cobalamin receptor) antibody
- Cubilin antibody
see all
图片
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Anti-Cubilin antibody [EPR16259-34] - C-terminal (ab191074) at 1/5000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 399 kDa
Observed band size: 399 kDa -
Western blot analysis of Cublin in immunoprecipitation pellets from Human fetal kidney lysate. ab191074 used at 1/40 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab191074 尚未被引用在任何文献中。