Application
ChIP
Sample
Human Cell lysate - nuclear (Lymphoblastoid cell line)
Negative control
No antibody control for ChIP. Negative region for qPCR.
Specification
Lymphoblastoid cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA 1%
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA 1%
Positive control
ChIP with anti-Pol2, anti-IRF4, anti-MEF2A, anti-RELA antibodies. The Possitive regions assessed by qPCR (IGLL5-Enh, LAPTM5-Enh) were validated using ENCODE data to be bound by studied TFs.
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: ChIP dilution buffer
Concentration
3 µg/µg chromatin
Additional data
Abcam response
The reviewer has used this antibody in a tested species and application. We have offered a refund or replacement as per our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 17 2016