重组Anti-CTGF抗体[EPR20728] (ab209780)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20728] to CTGF
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CTGF抗体[EPR20728]
参阅全部 CTGF 一抗 -
描述
兔单克隆抗体[EPR20728] to CTGF -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Starved NIH/3T3 cells treated with TGF-ß1 (ab50036) and Heparin sodium salt and HepG2 whole cell lysate. IP: HepG2 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20728 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab209780于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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IP |
1/30.
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说明 |
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WB
1/1000. Predicted molecular weight: 38 kDa. |
IP
1/30. |
靶标
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功能
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. -
组织特异性
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. -
序列相似性
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1490 Human
- Entrez Gene: 14219 Mouse
- Omim: 121009 Human
- SwissProt: P29279 Human
- SwissProt: P29268 Mouse
- Unigene: 410037 Human
- Unigene: 390287 Mouse
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别名
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
图片
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All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate at 20 µg
Lanes 2 & 4 : Empty
Lane 3 : CTGF knockout U-2 OS cell lysate at 20 µg
Lane 5 : HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDaFalse colour image of Western blot: Anti-CTGF antibody [EPR20728] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab209780 was shown to bind specifically to CTGF. A band was observed at 38 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in CTGF knockout cell line ab261880 (knockout cell lysate ab261689). To generate this image, wild-type and CTGF knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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CTGF was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab209780 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209780 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg (Input).
Lane 2: ab209780 IP in HepG2 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209780 in HepG2 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The molecular mass observed is consistent with the literature. (PMID:27126736)
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All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate
Lane 2 : NIH/3T3 starved for 18 hours, then treated with 10 ng/ml transforming growth factor-ß (TGF-ß1, ab50036) and 50 µg/ml Heparin sodium salt for 24 hours, whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
The level of CTGF expression can be induced by TGF beta treatment (PMID: 17786299).
CTGF is constitutively expressed in HepG2 cells (PMID:15886528).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (13)
ab209780 被引用在 13 文献中.
- Yamamoto A et al. Therapeutic Effect of Cyclin-Dependent Kinase 4/6 Inhibitor on Dermal Fibrosis in Murine Models of Systemic Sclerosis. Arthritis Rheumatol 74:860-870 (2022). PubMed: 34882985
- Sun L et al. PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II. Cell Rep 38:110349 (2022). PubMed: 35139382
- Deng W et al. Silencing lncRNA Snhg6 mitigates bleomycin-induced pulmonary fibrosis in mice via miR-26a-5p/TGF-β1-smads axis. Environ Toxicol 37:2375-2387 (2022). PubMed: 35785413
- Wang Y et al. A rare case of arrhythmogenic right ventricular cardiomyopathy associated with LAMA2 mutation: A case report and literature review. Front Med (Lausanne) 9:922347 (2022). PubMed: 35924034
- Shao Y et al. Ubiquitin-like protein FAT10 promotes renal fibrosis by stabilizing USP7 to prolong CHK1-mediated G2/M arrest in renal tubular epithelial cells. Aging (Albany NY) 14:7527-7546 (2022). PubMed: 36152057