Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
None
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: 488 Anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
We did not see any staining. We tried both with antigen retrieval and without antigen retrieval on multiple sections. Secondary antibody performed well with an alternative primary during the same staining run.
Abcam response
Results may be improved using methacarn fixation, similar to another user of this antibody. (http://www.cpl.colostate.edu/pathology/protocols/methacarn.htm).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 02 2018