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Anti-CTGF抗体(ab6992)

Reviews (27)Q&A (34)References (262)

Key features and details

  • Rabbit polyclonal to CTGF
  • Suitable for: IHC-P, ICC/IF, IHC-FoFr, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-CTGF antibody [EPR20728] (ab209780)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-CTGF抗体
    参阅全部 CTGF 一抗

  • 描述

    兔多克隆抗体to CTGF

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, ICC/IF, IHC-FoFr, WB, IHC-Fr, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Sheep, Human
    预测可用于: Cow

  • 免疫原

    Recombinant fragment corresponding to Mouse CTGF aa 223-348.
    Database link: P29268
    (Peptide available as ab7861)

  • 阳性对照

    • human hippocampal protein extracts (see Ueberham et al paper).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6992于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (10)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-FoFr (1)
Use at an assay dependent concentration.
WB (10)
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.
IHC-Fr (2)
Use at an assay dependent concentration.

See Ueberham et al.
IP
Use at an assay dependent concentration. PubMed: 16100286
说明
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.
IHC-Fr
Use at an assay dependent concentration.

See Ueberham et al.
IP
Use at an assay dependent concentration. PubMed: 16100286

靶标

  • 功能

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

  • 组织特异性

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

  • 序列相似性

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

文献 (262)

发表研究结果有使用 ab6992？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6992 被引用在 262 文献中.

  • Zhang Y  et al. miR-212 promotes renal interstitial fibrosis by inhibiting hypoxia-inducible factor 1-a inhibitor. Mol Med Rep 23:N/A (2021). PubMed: 33495813
  • Lyu L  et al. Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress. J Cell Mol Med 25:3136-3148 (2021). PubMed: 33560596
  • Zhu F  et al. Tubular Numb promotes renal interstitial fibrosis via modulating HIF-1a protein stability. Biochim Biophys Acta Mol Basis Dis 1867:166081 (2021). PubMed: 33486098
  • Tokuda K  et al. Development of a novel knee contracture mouse model by immobilization using external fixation. Connect Tissue Res N/A:1-14 (2021). PubMed: 33602048
  • Liu Y  et al. Inhibiting miR-1 attenuates pulmonary arterial hypertension in rats. Mol Med Rep 23:N/A (2021). PubMed: 33604679
View all Publications for this product

客户评价及客户问答

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1-10 of 61 Abreviews or Q&A

Western blot abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
30 µg
Treatment
particles
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Pureunhaneul Lee

Verified customer

提交于 Dec 25 2019

Western blot abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 17 2019

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 19 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
None
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (Tris/EDTA)
Permeabilization
No
Specification
Jejunum
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 19 2017

Western blot abreview for Anti-CTGF antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (iPSC derived nuerons)
Specification
iPSC derived nuerons
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 26 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (saphenous vein)
Specification
saphenous vein
Permeabilization
Yes - tween20
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 19 2014

Western blot abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (retina)
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 12 2014

Western blot abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 03 2014

Question

IHC-P with mouse skin no signal tried with a nd withour AR (citrate buffer, microwave) warm vial (was left out for 2 days on loading dock) used same Ab before and worked, different lot Ab: 1/100 - 1/1000 several dilutions, o/n 4 degree block with goat serum 10% + TX-100 secondary: fine

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

1-10 of 61 Abreviews or Q&A

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