Anti-CTGF抗体(ab5097)
Key features and details
- Rabbit polyclonal to CTGF
- Suitable for: ICC, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-CTGF抗体
参阅全部 CTGF 一抗 -
描述
兔多克隆抗体to CTGF -
宿主
Rabbit -
特异性
This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Rat, Human, Pig -
免疫原
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use a concentration of 1 µg/ml. | |
WB | 1/500. Predicted molecular weight: 38 kDa.Can be blocked with Human CTGF peptide (ab13741). This application has only been tested on recombinant protein. |
靶标
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功能
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. -
组织特异性
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. -
序列相似性
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1490 Human
- Entrez Gene: 100152141 Pig
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: P29279 Human
- SwissProt: O19113 Pig
- SwissProt: Q9R1E9 Rat
- Unigene: 410037 Human
see all -
别名
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
图片
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ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.
Not yet tested against endogenous CTGFSecondary antibody - anti-rabbit HRP (ab6721)
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Immunocytochemistry - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Beate Thode
ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).
文献 (14)
ab5097 被引用在 14 文献中.
- Li L et al. MicroRNA-584 Impairs Cellular Proliferation and Sensitizes Osteosarcoma Cells to Cisplatin and Taxanes by Targeting CCN2. Cancer Manag Res 12:2577-2587 (2020). PubMed: 32346311
- Sourbier C et al. Targeting loss of the Hippo signaling pathway in NF2-deficient papillary kidney cancers. Oncotarget 9:10723-10733 (2018). PubMed: 29535838
- Lambers E et al. Foxc1 Regulates Early Cardiomyogenesis and Functional Properties of Embryonic Stem Cell Derived Cardiomyocytes. Stem Cells 34:1487-500 (2016). PubMed: 26824887
- Lee CH et al. Harnessing endogenous stem/progenitor cells for tendon regeneration. J Clin Invest 125:2690-701 (2015). PubMed: 26053662
- Meng YH et al. Elevated expression of connective tissue growth factor, osteopontin and increased collagen content in human ascending thoracic aortic aneurysms. Vascular 22:20-7 (2014). IHC ; Human . PubMed: 23508392