- 数据表
- 具体的参考文献 (12)
- 实验方案
概述
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产品名称Anti-CTGF抗体
参阅全部 CTGF 一抗 -
描述兔多克隆抗体to CTGF
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宿主Rabbit
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特异性This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097.
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经测试应用适用于: IHC-P, WB, ICC/IF, IHC-Frmore details
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种属反应性与反应: Rat, Human, Pig
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免疫原
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阳性对照
- This antibody gave a positive signal in recombinant human CTGF in WB; This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab5097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 17028989 | |
| WB | 1/500. Predicted molecular weight: 38 kDa. This application has only been tested on recombinant protein. |
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| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-Fr | 1/200. |
靶标
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功能Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
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组织特异性Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
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序列相似性Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
细胞定位Secreted > extracellular space > extracellular matrix. Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 1490 Human
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: P29279 Human
- SwissProt: Q9R1E9 Rat
- Unigene: 410037 Human
- Unigene: 17145 Rat
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别名
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (ab5097)This image is courtesy of an anonymous Abreview.ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal. -
Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody (ab5097)ICC/IF image of ab5097 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab5097 staining CTGF in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Beate Thodeab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTGF antibody (ab5097)This image is courtesy of an anonymous Abreviewab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 24 hours at 4°C. A BHRP-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Gabrielle le Provostab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS Tween 0.01% + donkey serum 1%) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/500) was used as the secondary antibody. -
Western blot - Anti-CTGF antibody (ab5097)Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.
Not yet tested against endogenous CTGFSecondary antibody - anti-rabbit HRP (ab6721)
文献
This product has been referenced in:
- Sourbier C et al. Targeting loss of the Hippo signaling pathway in NF2-deficient papillary kidney cancers. Oncotarget 9:10723-10733 (2018). Read more (PubMed: 29535838) »
- Lee CH et al. Harnessing endogenous stem/progenitor cells for tendon regeneration. J Clin Invest 125:2690-701 (2015). Read more (PubMed: 26053662) »
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