Anti-CTBP2抗体- C-terminal (ab190933)
Key features and details
- Rabbit polyclonal to CTBP2 - C-terminal
- Suitable for: IHC-Fr, WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CTBP2抗体- C-terminal
参阅全部 CTBP2 一抗 -
描述
兔多克隆抗体to CTBP2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, WB, IHC-P, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Synthetic peptide corresponding to Human CTBP2 aa 400 to the C-terminus (C terminal).
Database link: P56545 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab190933于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 49 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 0.5 - 1 µg/ml.
|
说明 |
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 49 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 0.5 - 1 µg/ml. |
靶标
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功能
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
组织特异性
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
序列相似性
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
翻译后修饰
Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
细胞定位
Nucleus. Cell junction > synapse. - Information by UniProt
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数据库链接
- Entrez Gene: 281730 Cow
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 81717 Rat
- Omim: 602619 Human
- SwissProt: Q0VCQ1 Cow
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
see all -
别名
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
图片
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All lanes : Anti-CTBP2 antibody - C-terminal (ab190933) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lane 5 : COLO320 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : SW620 cell lysate
Predicted band size: 49 kDa -
CTBP2 was detected in frozen section of mouse intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in immunocytochemical section of human Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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CTBP2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190933 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab190933 尚未被引用在任何文献中。