重组Anti-CSRP3抗体[EPR12615(B)] (ab172952)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12615(B)] to CSRP3
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CSRP3抗体[EPR12615(B)]
参阅全部 CSRP3 一抗 -
描述
兔单克隆抗体[EPR12615(B)] to CSRP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide within Human CSRP3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P50461 -
阳性对照
- Fetal heart, human skeletal muscle, fetal muscle lysates, human heart tissue, human uterus tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12615(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172952于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
靶标
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功能
Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. -
组织特异性
Cardiac and slow-twitch skeletal muscles. -
疾病相关
Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M) [MIM:607482]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in CSRP3 are the cause of cardiomyopathy familial hypertrophic type 12 (CMH12) [MIM:612124]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. -
序列相似性
Contains 2 LIM zinc-binding domains. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Mainly cytoplasmic (By similarity). In the nucleus it associates with the actin cytoskeleton (Potential). In the Z line, found associated with GLRX3. - Information by UniProt
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数据库链接
- Entrez Gene: 540407 Cow
- Entrez Gene: 8048 Human
- Entrez Gene: 13009 Mouse
- Entrez Gene: 117505 Rat
- Omim: 600824 Human
- SwissProt: Q4U0T9 Cow
- SwissProt: P50461 Human
- SwissProt: P50462 Mouse
see all -
别名
- cardiac antibody
- Cardiac LIM protein antibody
- CLP antibody
see all
图片
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All lanes : Anti-CSRP3 antibody [EPR12615(B)] (ab172952) at 1/1000 dilution
Lane 1 : Fetal heart
Lane 2 : Human skeletal muscle
Lane 3 : Fetal muscle
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 21 kDa -
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling CSRP3 with ab172952 at 1/1000 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling CSRP3 with ab172592 at 1/1000 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal lung tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab172952 showing -ve staining in Human normal brain tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1X PBS (negative control) using ab172952, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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