Anti-CSK抗体(ab744)
概述
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产品名称Anti-CSK抗体
参阅全部 CSK 一抗 -
描述山羊多克隆抗体to CSK
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宿主Goat
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经测试应用适用于: WB, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Human
预测可用于: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human CSK aa 438-450 (C terminal).
Sequence:C-EQLEHIKTHELHL
(Peptide available asab19039) -
阳性对照
- Jurkat cell lysate, Human colon tissue.
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常规说明GenBank Accession Number – NP_004374.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human CSK peptide (ab19039). | |
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
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组织特异性Expressed in lung and macrophages.
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序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
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细胞定位Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
- Information by UniProt
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数据库链接
- Entrez Gene: 509246 Cow
- Entrez Gene: 487649 Dog
- Entrez Gene: 1445 Human
- Entrez Gene: 12988 Mouse
- Entrez Gene: 315707 Rat
- Omim: 124095 Human
- SwissProt: Q0VBZ0 Cow
- SwissProt: P41240 Human
see all -
别名
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
see all
图片
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Western blot - Anti-CSK antibody (ab744)ab744 staining (0.5µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. -
Western blot - Anti-CSK antibody (ab744)Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
数据表及文件
文献
This product has been referenced in:
- Yeh WL et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . Read more (PubMed: 23593342) »
- Ribeiro-Oliveira A et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . Read more (PubMed: 18701577) »
