Anti-CSK抗体(ab744)

Submit a review Q&A (1)References (2)
Western blot - Anti-CSK antibody (ab744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
  • Western blot - Anti-CSK antibody (ab744)

Key features and details

  • Goat polyclonal to CSK
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-CSK antibody [EPR6771] (ab125005)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Mouse
Human
All applications
Cow
Dog
应用 Ab评论 说明
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

1 hour primary incubation is recommended for this product.
IHC-P
Use at an assay dependent concentration.
说明
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

1 hour primary incubation is recommended for this product.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

  • 组织特异性

    Expressed in lung and macrophages.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 翻译后修饰

    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.

  • 细胞定位

    Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • C SRC antibody
    • C SRC kinase antibody
    • C src Tyrosine Kinase antibody
    • C-SRC kinase antibody
    • c-src tyrosine kinase antibody
    • Csk A antibody
    • CSK antibody
    • CSK_HUMAN antibody
    • CYTOPLASMIC TYROSINE KINASE antibody
    • EC 2.7.10.2 antibody
    • MGC112926 antibody
    • MGC117393 antibody
    • MGC154049 antibody
    • P60 Src antibody
    • Protein tyrosine kinase CYL antibody
    • Protein-tyrosine kinase CYL antibody
    • Proto oncogene tyrosine protein kinase antibody
    • Tyrosine protein kinase CSK antibody
    • Tyrosine-protein kinase CSK antibody
    • zgc:154049 antibody
    see all

图片

  • Western blot - Anti-CSK antibody (ab744)
    Western blot - Anti-CSK antibody (ab744)
    ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)
    ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Western blot - Anti-CSK antibody (ab744)
    Western blot - Anti-CSK antibody (ab744)
    Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa

文献 (2)

发表研究结果有使用 ab744？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab744 被引用在 2 文献中.

  • Yeh WL  et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . PubMed: 23593342
  • Ribeiro-Oliveira A  et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . PubMed: 18701577

客户评价及客户问答

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Question

Has this antibody ever been tested on any samples other than Jurkat lysate?

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Answer

Thank you for your enquiry. I contacted the originator of this antibody and they said they also did a preliminary Western blot with mouse spleen lysate and saw a band at approximately 45 kDa - but they aren't sure if it's correct. Please let me know if you need additional assistance.

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