存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab1302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 33 kDa.|
|ICC||Use a concentration of 2.5 - 3.8 µg/ml.|
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent concentration.|
功能May mediate the transduction of intracellular signals.
序列相似性Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
Anti-CrkL antibody (ab1302) at 0.01 µg/ml + K562 lysate (35µg protein in RIPA buffer).
Predicted band size: 33 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab1302 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab1302 has not yet been referenced specifically in any publications.