Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse, whole brain sections)
Specification
Mouse, whole brain sections
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Agent
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFlour 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Brains were perfused with 4% paraformaldehyde.
Sections cut and stained at 20 microns for immunohistochemistry on slides.
Blocking agent also has 0.5% fish gelatin.
This antibody produced very little background and no bleed-through. It was a strong nuclear stain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 13 2014