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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-CREBBP抗体(ab2832)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (48)

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Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
  • Western blot - Anti-CREBBP antibody (ab2832)

Key features and details

  • Rabbit polyclonal to CREBBP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-CREBBP抗体
    参阅全部 CREBBP 一抗
  • 描述

    兔多克隆抗体to CREBBP
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Non human primates
  • 免疫原

    Synthetic peptide corresponding to Human CREBBP aa 162-176.
    Sequence:

    ATSSPATSQTGPGIC


    (Peptide available as ab4916)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CREBBP protein (ab167964)

应用

Our Abpromise guarantee covers the use of ab2832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).
IHC-P 1/1000 - 1/5000.
ICC/IF 1/100.

靶标

  • 功能

    Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
  • 疾病相关

    Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
  • 序列相似性

    Contains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • 结构域

    The KIX domain mediates binding to HIV-1 Tat.
  • 翻译后修饰

    Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
  • 细胞定位

    Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
  • Target information above from: UniProt accession Q92793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1387 Human
    • Entrez Gene: 12914 Mouse
    • Entrez Gene: 54244 Rat
    • Omim: 600140 Human
    • SwissProt: Q92793 Human
    • SwissProt: P45481 Mouse
    • SwissProt: Q6JHU9 Rat
    • Unigene: 459759 Human
    • Unigene: 132238 Mouse
    • Unigene: 392384 Mouse
    • Unigene: 12815 Rat
    see all
  • 别名

    • CBP antibody
    • CBP_HUMAN antibody
    • CREB binding protein antibody
    • CREB-binding protein antibody
    • Crebbp antibody
    • Cyclic AMP responsive enhancer binding protein antibody
    • KAT3A antibody
    • RSTS antibody
    • RTS antibody
    • Rubinstein Taybi syndrome antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
    Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)

    Immunofluorescent analysis of MCF-7 cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)

    IHC image of CREBBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2832, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
    Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)

    Immunofluorescent analysis of HeLa cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)

    Immunohistochemical analysis of FFPE mouse colon tissue, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Heat antigen retrieval performed with 10mM Sodium Citrate (pH 6) for 8-15 minutes. Tissue blocked with 3% H2O2-methanol for 15 minutes at room temperature. Incubation with ab2832 diluted 1/2000 in 3% BSA-PBS overnight at 4°C. Counterstaining with hematoxylin. 

  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
    Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)

    Immunofluorescent analysis of NIH-3T3 cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Nuclei were stained with DAPI (blue).

  • Western blot - Anti-CREBBP antibody (ab2832)
    Western blot - Anti-CREBBP antibody (ab2832)
    ab2832 using HeLa cell lysate. ab2832 using HeLa cell lysate.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (48)

    发表研究结果有使用 ab2832?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2832 被引用在 48 文献中.

    • Lee W  et al. Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor ß-induced protein and improves survival in sepsis. Biomaterials 246:120000 (2020). PubMed: 32247936
    • Eldridge MJG  et al. Active nuclear import of the deacetylase Sirtuin-2 is controlled by its C-terminus and importins. Sci Rep 10:2034 (2020). PubMed: 32042025
    • Xian D & Zhao Y LncRNA KCNQ1OT1 enhanced the methotrexate resistance of colorectal cancer cells by regulating miR-760/PPP1R1B via the cAMP signalling pathway. J Cell Mol Med 23:3808-3823 (2019). PubMed: 30997746
    • Ding H  et al. Systematic Analysis of Drug Vulnerabilities Conferred by Tumor Suppressor Loss. Cell Rep 27:3331-3344.e6 (2019). PubMed: 31189115
    • Dong H  et al. Activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast Cancer. Mol Cancer 18:3 (2019). PubMed: 30621694
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-KAT3A / CBP antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole brain tissue lysate)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
    Loading amount
    20 µg
    Specification
    Whole brain tissue lysate
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 31 2017

    ChIP abreview for Anti-KAT3A / CBP antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Mouse Tissue lysate - nuclear (Liver)
    Specification
    Liver
    Negative control
    no ab
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: PFA 1%
    Positive control
    ChIP with anti-Pol ab
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 16 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KAT3A / CBP antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Mouse, whole brain sections)
    Specification
    Mouse, whole brain sections
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 13 2014

    Western blot abreview for Anti-KAT3A / CBP antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chicken Tissue lysate - whole (brain)
    Loading amount
    5.2 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Ms. Ying Jiang

    Verified customer

    提交于 Sep 21 2012

    Question

    Inquiry: Hello, I am looking for a CBP antibody for use in the ChIP assay in human cells. I have noted that the ab2832 antibody has been used in ChIP. However, I require the antibody to be specific to CBP and to NOT cross-react with p300, since I wish to differentiate between both. The two proteins are very similar to each other and are between 80-90% homologous in some of the protein binding regions. Has the a b2832 antibody been tested to be specific for CBP and not cross-react with p300? Thank you, Sushma

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2012

    Answer

    Thank you for your enquiry.
    The exact immunogen sequence used to generate ab2832 is noted on the online product datasheet as:
    Synthetic peptide: ATSSPATSQTGPGIC, corresponding to amino acids 162-176 in the nuclear factor binding domain of Human CBP.
    From the webpage you can do a BLAST via NCBI. I have done this search and no p300 protein hits are generated. If you wish you can also align p300 to this peptide to ensure there are no large regions of identity between the 2 sequences.
    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 Jul 12 2012

    Question

    I am looking for a CBP or P300 antibody to use ChIP assay using zebrafish embryos. What would you recommend from your antibody list? Please let me know if you find any. Thank you so much in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 08 2011

    Answer

    Thank you for contacting us. We have the following antibodies in our catalog that where tested in ChIP: for CBP: ab2832, ab10489, and ab10490 for p300: ab14984 None of the antibodies has been tested so far in zebrafish. If you could send me please the protein sequences for zebrafish, then I can check the homology. If the homology is about 85% or higher, you can participate in our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Please let me know the zebrafish sequence and I can check which of the antibodies might be suitable to try in this species. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Sep 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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