为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Signal Transduction Second Messenger Nucleotide Messenger cAMP

Anti-CREB + ICER抗体(ab5803)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (11)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-CREB + ICER antibody (ab5803)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + ICER antibody (ab5803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + ICER antibody (ab5803)
  • Western blot - Anti-CREB + ICER antibody (ab5803)

Key features and details

  • Rabbit polyclonal to CREB + ICER
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

你可能会感兴趣

二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一抗
Product image
Anti-CREB (phospho S133) antibody [E113] - BSA and Azide free (ab220798)
一抗
Product image
Anti-CREB (phospho S133) antibody [E113] (ab32096)

查看更多相关产品

概述

  • 产品名称

    Anti-CREB + ICER抗体
  • 描述

    兔多克隆抗体to CREB + ICER
  • 宿主

    Rabbit
  • 特异性

    ab5803 detects both the phosphorylated and non-phosphorylated forms of cyclic-AMP response element binding protein (CREB) from rat cells.
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human CREB aa 123-136.
    Sequence:

    KRREILSRRPSYRK


    (Peptide available as ab5860)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Immunology
    • Innate Immunity
    • TLR Signaling

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • CREB + ICER peptide (ab5860)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5803于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use a concentration of 2 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with CREB + ICER peptide (ab5860).
ICC/IF (1)
1/50 - 1/500.
IHC-P
1/20 - 1/200.
说明
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with CREB + ICER peptide (ab5860).
ICC/IF
1/50 - 1/500.
IHC-P
1/20 - 1/200.

靶标

  • 细胞定位

    CREB: Nucleus. CREM: Nucleus.
  • 数据库链接

    • Entrez Gene: 281713 Cow
    • Entrez Gene: 540605 Cow
    • Entrez Gene: 403887 Dog
    • Entrez Gene: 1385 Human
    • Entrez Gene: 1390 Human
    • Entrez Gene: 12912 Mouse
    • Entrez Gene: 12916 Mouse
    • Entrez Gene: 25620 Rat
    • Entrez Gene: 81646 Rat
    • Omim: 123810 Human
    • Omim: 123812 Human
    • SwissProt: P27925 Cow
    • SwissProt: Q1LZH5 Cow
    • SwissProt: P79145 Dog
    • SwissProt: P16220 Human
    • SwissProt: Q03060 Human
    • SwissProt: P27699 Mouse
    • SwissProt: Q01147 Mouse
    • SwissProt: P15337 Rat
    • SwissProt: Q03061 Rat
    • Unigene: 200250 Human
    • Unigene: 516646 Human
    • Unigene: 453295 Mouse
    • Unigene: 5244 Mouse
    • Unigene: 10251 Rat
    • Unigene: 90061 Rat
    see all

图片

  • Western blot - Anti-CREB + ICER antibody (ab5803)
    Western blot - Anti-CREB + ICER antibody (ab5803)

    Shows a Western blot of CREB on GH4 cell extract using ab5803.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + ICER antibody (ab5803)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB + ICER antibody (ab5803)

    Immunofluorescent analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)

    Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)

    Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded Human glioma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + ICER antibody (ab5803)

    Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded Human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + ICER antibody (ab5803)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB + ICER antibody (ab5803)

    Immunofluorescent analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Western blot - Anti-CREB + ICER antibody (ab5803)
    Western blot - Anti-CREB + ICER antibody (ab5803)This image is courtesy of Richard D'Mello, Kings College, London
    All lanes : Anti-CREB + ICER antibody (ab5803) at 1/500 dilution

    All lanes : hippocampal lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IR800-linked conjugated to IRDye 800CW at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 55 kDa (possible non-specific binding)


    Exposure time: 5 minutes

实验方案

  • Protocol for perfusion fixed tissue IHC (DAB)

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (11)

发表研究结果有使用 ab5803?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5803 被引用在 11 文献中.

  • Alves-Wagner AB  et al. Sympathetic Regulation of Slc2a4 Gene Expression: Participation of a Putative cAMP Responsive Element (CRE) Site in the Slc2a4 Promoter. Cell Physiol Biochem 52:580-594 (2019). PubMed: 30897323
  • Reddy NR & Krishnamurthy S Repeated olanzapine treatment mitigates PTSD like symptoms in rats with changes in cell signaling factors. Brain Res Bull 140:365-377 (2018). PubMed: 29902501
  • von Schalburg KR  et al. Subcellular localization and characterization of estrogenic pathway regulators and mediators in Atlantic salmon spermatozoal cells. Histochem Cell Biol N/A:N/A (2017). PubMed: 28983690
  • Kang X  et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . PubMed: 25915125
  • Singh SK  et al. GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nat Commun 4:1830 (2013). Rat . PubMed: 23652018
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-CREB + CREM antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
Sample
Salmo salar Cell (testicular)
Specification
testicular
Permeabilization
Yes - methyl methacrylate/Butyl methacrylate
Fixative
glutaraldehyde-formaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 25 2015

Western blot abreview for Anti-CREB antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Cow Tissue lysate - whole (Subcutaneous Adipose)
Specification
Subcutaneous Adipose
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Nov 08 2013

Question

Dear abcam, Would it be possible to receive free aliquots of your ab5803 and ab10564? My lab studies signal transduction pathways in aquatic animals (sharks, oysters, coral, etc), and are looking for commercial antibodies against CREB and P-CREB. If these antibodies work well with our samples we'd purchase them on a regular basis. thanks

Read More

Abcam community

Verified customer

Asked on May 23 2012

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Abcam Scientific Support

回复于 May 23 2012

Western blot abreview for Anti-CREB antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount
40 µg
Specification
Hippocampus lysate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Mr. Richard D'Mello

Verified customer

提交于 Sep 27 2010

Question

Recently, I bought 5 antibodies from abcam, which are: 1 x GABARAPL2 antibody(ab15858) 1 x CREB antibody (ab5803) 1 x Leptin Receptor antibody (ab7211) 1 x GABARAP antibody (ab1133) 1 x Chicken IgY (H&L) antibody (ab6753) The order reference number is 66504 (PON: L-15) I receive them yesterday and 3 of them were OK, but I think that there was some kind of trouble with two of them, GABARAPL2 antibody (ab15858) and GABARAP antibody (ab1133). I was expected to receive 50 uL of GABARAPL2 antibody and I only received 40 uL. In the case of GABARAP antibody I was expected to recieve 100 uL and I have only received 40 uL. In this case, the recomended dilution for this antibody is 1:500, so I probably won't be able to establish the optimal assay conditions for that antibody with the supplied amount of it. I write you to ask for a solution for this event, because I have paid for a determined amount of antibody and I have received less than the half.

Read More

Abcam community

Verified customer

Asked on Jan 28 2005

Answer

Le pido que nos disculpe por la falta en su orden. He hecho una nueva orden cual le sera enviada lo mas pronto posible. Porfavor refiera al numero de orden 68450 si necesita mas información sobre el estado de su orden. Otra vez, porfavor disculpe la falta que recibio. I offer you my sincerest apologies for the shortage you've received. I have gone ahead and set up a replacement order to be shipped to you as soon as possible. Please reference order number 68450 if you require further information about the status of your order. Once again, I apologize for the shortage you received.

Read More

Abcam Scientific Support

回复于 Jan 31 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2021 艾博抗(上海)贸易有限公司版权所有