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Signal Transduction Second Messenger Nucleotide Messenger cAMP

Anti-CREB + CREM抗体(ab5803)

  • Datasheet
Reviews (3)Q&A (2)References (11)

Product price, shipping and contact information

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Western blot - Anti-CREB + CREM antibody (ab5803)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + CREM antibody (ab5803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)
  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + CREM antibody (ab5803)
  • Western blot - Anti-CREB + CREM antibody (ab5803)

Key features and details

  • Rabbit polyclonal to CREB + CREM
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-CREB + CREM抗体
  • 描述

    兔多克隆抗体to CREB + CREM
  • 宿主

    Rabbit
  • 特异性

    ab5803 detects both the phosphorylated and non-phosphorylated forms of cyclic-AMP response element binding protein (CREB) from rat cells.
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human CREB aa 123-136.
    Sequence:

    KRREILSRRPSYRK


    (Peptide available as ab5860)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Immunology
    • Innate Immunity
    • TLR Signaling

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • CREB + CREM peptide (ab5860)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab5803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with CREB + CREM peptide (ab5860).
ICC/IF 1/50 - 1/500.
IHC-P 1/20 - 1/200.

靶标

  • 细胞定位

    CREB: Nucleus. CREM: Nucleus.
  • 数据库链接

    • Entrez Gene: 281713 Cow
    • Entrez Gene: 540605 Cow
    • Entrez Gene: 403887 Dog
    • Entrez Gene: 1385 Human
    • Entrez Gene: 1390 Human
    • Entrez Gene: 12912 Mouse
    • Entrez Gene: 12916 Mouse
    • Entrez Gene: 25620 Rat
    • Entrez Gene: 81646 Rat
    • Omim: 123810 Human
    • Omim: 123812 Human
    • SwissProt: P27925 Cow
    • SwissProt: Q1LZH5 Cow
    • SwissProt: P79145 Dog
    • SwissProt: P16220 Human
    • SwissProt: Q03060 Human
    • SwissProt: P27699 Mouse
    • SwissProt: Q01147 Mouse
    • SwissProt: P15337 Rat
    • SwissProt: Q03061 Rat
    • Unigene: 200250 Human
    • Unigene: 516646 Human
    • Unigene: 453295 Mouse
    • Unigene: 5244 Mouse
    • Unigene: 10251 Rat
    • Unigene: 90061 Rat
    see all

图片

  • Western blot - Anti-CREB + CREM antibody (ab5803)
    Western blot - Anti-CREB + CREM antibody (ab5803)

    Shows a Western blot of CREB on GH4 cell extract using ab5803.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + CREM antibody (ab5803)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB + CREM antibody (ab5803)

    Immunofluorescent analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)

    Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)

    Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded Human glioma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB + CREM antibody (ab5803)

    Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded Human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB + CREM antibody (ab5803)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB + CREM antibody (ab5803)

    Immunofluorescent analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Western blot - Anti-CREB + CREM antibody (ab5803)
    Western blot - Anti-CREB + CREM antibody (ab5803)This image is courtesy of Richard D'Mello, Kings College, London
    All lanes : Anti-CREB + CREM antibody (ab5803) at 1/500 dilution

    All lanes : hippocampal lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IR800-linked conjugated to IRDye 800CW at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 55 kDa (possible non-specific binding)


    Exposure time: 5 minutes

实验方案

  • Protocol for perfusion fixed tissue IHC (DAB)

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (11)

    发表研究结果有使用 ab5803?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5803 被引用在 11 文献中.

    • Alves-Wagner AB  et al. Sympathetic Regulation of Slc2a4 Gene Expression: Participation of a Putative cAMP Responsive Element (CRE) Site in the Slc2a4 Promoter. Cell Physiol Biochem 52:580-594 (2019). PubMed: 30897323
    • Reddy NR & Krishnamurthy S Repeated olanzapine treatment mitigates PTSD like symptoms in rats with changes in cell signaling factors. Brain Res Bull 140:365-377 (2018). PubMed: 29902501
    • von Schalburg KR  et al. Subcellular localization and characterization of estrogenic pathway regulators and mediators in Atlantic salmon spermatozoal cells. Histochem Cell Biol N/A:N/A (2017). PubMed: 28983690
    • Kang X  et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . PubMed: 25915125
    • Singh SK  et al. GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nat Commun 4:1830 (2013). Rat . PubMed: 23652018
    View all Publications for this product

    客户评价及客户问答

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    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CREB + CREM antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
    Sample
    Salmo salar Cell (testicular)
    Specification
    testicular
    Permeabilization
    Yes - methyl methacrylate/Butyl methacrylate
    Fixative
    glutaraldehyde-formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 25 2015

    Western blot abreview for Anti-CREB antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Cow Tissue lysate - whole (Subcutaneous Adipose)
    Specification
    Subcutaneous Adipose
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 08 2013

    Question

    Dear abcam, Would it be possible to receive free aliquots of your ab5803 and ab10564? My lab studies signal transduction pathways in aquatic animals (sharks, oysters, coral, etc), and are looking for commercial antibodies against CREB and P-CREB. If these antibodies work well with our samples we'd purchase them on a regular basis. thanks

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2012

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    回复于 May 23 2012

    Western blot abreview for Anti-CREB antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Hippocampus lysate)
    Loading amount
    40 µg
    Specification
    Hippocampus lysate
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Richard D'Mello

    Verified customer

    提交于 Sep 27 2010

    Question

    Recently, I bought 5 antibodies from abcam, which are: 1 x GABARAPL2 antibody(ab15858) 1 x CREB antibody (ab5803) 1 x Leptin Receptor antibody (ab7211) 1 x GABARAP antibody (ab1133) 1 x Chicken IgY (H&L) antibody (ab6753) The order reference number is 66504 (PON: L-15) I receive them yesterday and 3 of them were OK, but I think that there was some kind of trouble with two of them, GABARAPL2 antibody (ab15858) and GABARAP antibody (ab1133). I was expected to receive 50 uL of GABARAPL2 antibody and I only received 40 uL. In the case of GABARAP antibody I was expected to recieve 100 uL and I have only received 40 uL. In this case, the recomended dilution for this antibody is 1:500, so I probably won't be able to establish the optimal assay conditions for that antibody with the supplied amount of it. I write you to ask for a solution for this event, because I have paid for a determined amount of antibody and I have received less than the half.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 28 2005

    Answer

    Le pido que nos disculpe por la falta en su orden. He hecho una nueva orden cual le sera enviada lo mas pronto posible. Porfavor refiera al numero de orden 68450 si necesita mas información sobre el estado de su orden. Otra vez, porfavor disculpe la falta que recibio. I offer you my sincerest apologies for the shortage you've received. I have gone ahead and set up a replacement order to be shipped to you as soon as possible. Please reference order number 68450 if you require further information about the status of your order. Once again, I apologize for the shortage you received.

    Read More

    Abcam Scientific Support

    回复于 Jan 31 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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