Anti-Creatine Kinase MB抗体[CK1] (ab404)

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Key features and details

  • Mouse monoclonal [CK1] to Creatine Kinase MB
  • Suitable for: ELISA, RIA
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Creatine Kinase MB抗体[CK1]
    参阅全部 Creatine Kinase MB 一抗

  • 描述

    小鼠单克隆抗体[CK1] to Creatine Kinase MB

  • 宿主

    Mouse

  • 特异性

    CK1 recognises human creatine kinase MB isoenzyme. Its affinity for the creatine kinase BB isoenzyme is greater than its affinity for creatine kinase MB isoenzyme. hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x109 l/mol human CK-MB.

  • 经测试应用

    适用于: ELISA, RIAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native protein (purified) (Human).

性能

应用

Our Abpromise guarantee covers the use of ab404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

靶标

  • 相关性

    Creatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.

  • 细胞定位

    Cytoplasmic and Mitochondrial

  • 数据库链接

    • 别名

      • B-CK antibody
      • brain creatine kinase antibody
      • CKBB antibody
      • CKMB antibody
      • CKMM antibody
      • creatine kinase B antibody
      • creatine kinase brain antibody
      • creatine kinase M antibody
      • Creatine kinase MB antibody
      • creatine kinase muscle antibody
      • M-CK antibody
      • muscle creatine kinase antibody
      see all

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献

    发表研究结果有使用 ab404？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab404 被引用在 2 文献中.

    • Garay F  et al. Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples. Anal Bioanal Chem 397:1873-81 (2010). PubMed: 20449577
    • Crawford RM  et al. Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo. FASEB J 16:102-4 (2002). PubMed: 11729098

    客户评价及客户问答

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    Question

    I used the mouse monoclonal [CK1] to Creatine Kinase MB (ab404) from Abcam. I have some question to ask you. I would like to know isoelectric point (pI) of this antibody for my applications. I look forward to hearing answer from you. Thank you very much and best regards,

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    Answer

    Thank you for your enquiry. I can confirm the pI range for ab404 Creatine Kinase MB antibody [CK1] is 6.9 to 8.3. Antibodies have ranges of pI rather than points. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    I\'d like to know if this product can be used to detect mouse creatine kinase B in immunoistochemistry experiments? (free floating sections of fixed spinal cord. Thank you for your attention

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    Answer

    All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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    Question

    Is this antibody suitable for Immunoprecipitation from TritonX 100 lysates? If yes, what dilutions do you suggest

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    Answer

    This antibody has not been tested in immunoprecipitation, so we can't guarantee that it will work.

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    Question

    Is this antibody suitable for western blotting?

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    Answer

    Dear Gary Please see my answer to your question on ab8364. This is essentially repeated below. This antibody hasn't been tested in Westerns. It is a monoclonal and it works in ELISA and Radioimmunoassays. It is possible that it works in Westerns and I would expect it to work in native gels, however the epitope it recognises may be dependent on tertiary structure and if this is the case it probably wouldn't work in denaturing gels. In general, I believe that ELISA and Radioimmunoassays are performed using native protein. I hope this helps. Regards Michael

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    Question

    Having bought the above product, I need to know a rough dilution value for Western bloting and ELISA. Please suggest a good starting point. Thanks. R. Crawford

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    Answer

    This antibody has not been tested in Western blotting applications, but a good starting dilution for both ELISA and Western would be 1/1000.

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    Question

    Does this antibody recognize mouse CK-MB?

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    Answer

    This antibody has not been tested for cross-reactivity against mouse. However as it was raised in mouse, cross-reactivity against the mouse antigen is unlikely.

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