The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
Cyclization recombinase antibody
Recombinase cre antibody
Western blot - Anti-Cre recombinase antibody (ab40011)This image is courtesy of Britta Eickholt, Univ London Kings Coll, United Kingdom
All lanes : Anti-Cre recombinase antibody (ab40011) at 1/100 dilution
Lane 1 : Hek293 cell lysate expressing Cre-recombinase Lane 2 : Control vector
Lysates/proteins at 10 µg per lane.
Secondary All lanes : goat-anti Rabbit HRP conjugated at 1/3000 dilution