• 产品名称
    Anti-Cre recombinase抗体[7.23]
    参阅全部 Cre recombinase 一抗
  • 描述
    小鼠单克隆抗体[7.23] to Cre recombinase
  • 宿主
  • 特异性
    Current Batch not validated for Immunohistochemistry.
  • 经测试应用
    适用于: WBmore details
  • 免疫原

    Full length native protein (affinity purified).

  • 常规说明

    This antibody can be used to determine the success of gene manipulations in transgenic animals.


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    This antibody can be used to determine the success of gene manipulations in transgenic animals.
  • 克隆
  • 克隆编号
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab24607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).


  • 相关性
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • 别名
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody


  • All lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution

    Lane 1 : Escherichia coli expressing Cre-recombinase
    Lane 2 : Hek cell expressing control plasmid

    Lysates/proteins at 10 µg per lane.

    All lanes : goat anti-mouse HRP conjugated at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50&80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 minute



This product has been referenced in:
  • Chen E  et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). Read more (PubMed: 29371319) »
  • Wu Y  et al. Ga12 is required for renal cystogenesis induced by Pkd1 inactivation. J Cell Sci 129:3675-3684 (2016). Read more (PubMed: 27505895) »
See all 15 Publications for this product


1-10 of 11 Q&A


Thank you for contacting us.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Thank you for contacting us.

Please check the following;


It is also used in other publications but we are unable to confirm the applications due to non availability of access to these articles.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Ante todo quisiera desearle un muy Feliz Año.

Muchas gracias por tomarte la molestia de rellenar el cuestionario y mandar más imágenes.

Hay alguna sugerencia que posiblemente mejore la tinción obtenida. Me decías en el primer correo que se había probado ya la recuperación antigénica del tejido tras fijación con 4%PFA durante la noche. Es posible que fijando las secciones congeladas con acetona a temperatura ambiente durante 10 minutos se observe mayor tinción. Además, la acetona tiene efecto permeabilizador en las células, facilitando la inclusión del anticuerpo en las mismas.

Otra recomendación que quisiera hacer es probar de bloquear el tejido de forma más suave. Se está utilizando un porcentaje muy alto de suero (16%), y los anticuerpos además se diluyen en el mismo.

Si podéis usar una dilución menos concentrada (10%) y diluir los anticuerpos en TBST con un porcentaje bajo (1%) de BSA, quizás la membrana no quede tan bloqueada y se permita una mejor visualización de la tinción.

En cualquier caso, si preferís que apliquemos directamente la garantía, puesto que ya habíais realizado pasos de optimización, y que os mandemos un reemplazo del anticuerpo (del mismo u otro diferente) o reembolso del importe del mismo, no dudes en indicármelo y estaré encantada de poder ayudar.

Espero entonces tu respuesta para proceder y quedo a la disposición de cualquier otra consulta que podáis tener.

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Thank you for contacting us.

Perhaps these are the antibodies customer is looking for;




I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Ich möchte Ihnen hiermit nochmals viel Erfolg wünschen und drücke die Daumen, dass dieser Antikörper funktioniert.

Wenn Sie Ihre Versuche abgeschlossen haben würden wir uns über einen Abreview freuen. Die gesammelten Abpoints können Sie für weitere Abcamprodukte einlösen oder auch in Amazongutscheine eintauschen. Wenn Sie mehr über unser Abreviewsystem erfahren wollen schauen Sie bitte https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews.

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Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie in Frage kommen und die Sie sich genauer anschauen sollten.

1. https://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

Immunogensequenz ist das gesamte native Cre Rekombinase Protein. Die Sequenz sollte daher mit der Sequenz in dem rekombinanten Protein übereinstimmen.

2. https://www.abcam.com/cre-recombinase-antibody-ab137240.html

Bei dem Immunogen handelt es sich um ein rekombinantes full length protein des Bacteriophage P1 Cre recombinase.

3. https://www.abcam.com/progesterone-receptor-antibody-alpha-pr6-ab2765.html

Die Immunogensequenz dieses Antikörpers ist leider nicht näher bestimmt. Der Antikörper wurde gegen das gesamte Hühnchen Progesteron gerichtet hergestellt. Die Hühnchensequenz verglichen mit der zur Verfügung gestellten Progesteronsequenz des rekombinanten Proteins weißt eine Uebereinstimmung von 78% auf. Die Detektion eines Proteins werten wir als wahrscheinlich, wenn eine Übereinstimmung von etwa 85% besteht.

Der ab24607 ist unter diesen drei Möglichkeiten der mit den meisten Referenzen und eingereichten Abreviews und daher am besten charakterisiert. Ab24607 wurde schon für IHC-Fr in Maus verwendet und zwei Abreviews speziell hierfür wurden verfasst.

Ich hoffe ich konnte Ihnen etwas weiter helfen. Es tut mir Leid dass ich Ihnen in diesem Fall kein perfektes Produkt anbieten kann.

Sollten Sie sich dafür entscheiden einen dieser Antikörper zu kaufen, möchte ich Sie noch auf unsere RabMab Aktion aufmerksam machen. Bitte lesen Sie mehr über diese Aktionhttps://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Bitte zögern Sie nicht sich wieder bei mir zu melden, sollten Sie weitere Fragen oder Probleme mit unseren Produkten haben.

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Thank you for contacting us.

We have 2 antibodies in our catalogwhich have beentested in IHC-Fr: ab24607, ab24580 (biotinylated).


As long as cre-recombinase is expressed in the cells or tissue - independent of the species of the samples - the anti-cre antibodies will work and are guaranteed in the applications listed as tested on the datasheet.

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

If you like to try any of the other cre-antibodies that have not yet tested in IHC-Fr (ab40011, ab10171, ab41104), in that case you would be eligible for our testing discount program:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

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Thank you for your reply.

I am happy to offer a replacement from another lot or a credit for this antibody. Please let me know which you would prefer.

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Thank you for contacting Abcam regarding ab24607.

I am sorry that you have been experiencing difficulties with this antibody in IHC. Based on the information you provided, I agree that this antibody is not performing as stated on the datasheet. Would you please provide an order number for your purchase? If you are contacting us within 6 months of purchase, you are eligible for a replacement or credit per our Abpromise guarantee.

I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

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Thank you for contacting us. The isotype is IgG1. We have 2 polyclonal; available ab40011 and ab41104 and one monoclonal which is ab101071 is IgM isotype. Please have a look at the datasheets and select the product as per your requirements. We unfortunately do not have exactly same product as ab24607. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 11 Q&A