参阅全部 Cre recombinase 一抗
描述小鼠单克隆抗体[7.23] to Cre recombinase
特异性Current Batch not validated for Immunohistochemistry.
经测试应用适用于: WBmore details
Full length native protein (affinity purified).
This antibody can be used to determine the success of gene manipulations in transgenic animals.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.03% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol
Concentration information loading...
纯度Protein G purified
Primary antibody说明This antibody can be used to determine the success of gene manipulations in transgenic animals.
Our Abpromise guarantee covers the use of ab24607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).|
相关性Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
- Cre antibody
- Cyclization recombinase antibody
- Recombinase cre antibody
All lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution
Lane 1 : Escherichia coli expressing Cre-recombinase
Lane 2 : Hek cell expressing control plasmid
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-mouse HRP conjugated at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50&80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
This product has been referenced in:
- Chen E et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). Read more (PubMed: 29371319) »
- Wu Y et al. Ga12 is required for renal cystogenesis induced by Pkd1 inactivation. J Cell Sci 129:3675-3684 (2016). Read more (PubMed: 27505895) »