Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (T84 epithelial cells)
Total protein in input
20 µg
Immuno-precipitation step
Protein A
Specification
T84 epithelial cells
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Triton lysis buffer
Dilution
1/1000
Western Blot antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: CAR Antibody (Mab.E[mh] 1), sc-32795
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Image shows immunoprecipitation of CAR with this product from whole cell lysate of T84 cells using protein A beads. Lysates were precleared for 1 hour before IP. CAR was visualized by western blotting with a mouse monoclonal antibody. A secondary HRP-conjugated antibody was used. IgG IP and Lysate Input are shown as controls.
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提交于 Dec 11 2021