Application
Western blot
Sample
Mouse Tissue lysate - whole (Small intestine)
Loading amount
50 µg
Specification
Small intestine
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-0.05% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: Horse anti-goat HRP
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 69 kDa Non-specific: None kDa
Positive control
Small intestinal extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 10 2012