Anti-COX2 / Cyclooxygenase 2抗体(ab23672)

概述

  • 产品名称
    Anti-COX2 / Cyclooxygenase 2抗体
    参阅全部 COX2 / Cyclooxygenase 2 一抗
  • 描述
    山羊多克隆抗体to COX2 / Cyclooxygenase 2
  • 宿主
    Goat
  • 特异性
    ab23672 recognises COX2. It does not cross react with COX1.
  • 经测试应用
    适用于: WB, IHC-P, ICC, Flow Cyt, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human
    预测可用于: Horse, Cow, Dog, Pig, Mink
  • 免疫原

    Synthetic peptide:

    TINASSSRSGLDDINPTVL

    , corresponding to amino acids 578/596 of Human COX2/ Cyclooxygenase 2(Peptide available as ab49841.)

应用

Our Abpromise guarantee covers the use of ab23672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab49841).
IHC-P Use a concentration of 1 - 2 µg/ml. Do not perform antigen retrieval.
ICC Use a concentration of 1 - 2 µg/ml.
Flow Cyt 1/600.

needs permeabilization

 

 

 

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/500. PubMed: 19632905

靶标

  • 功能
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • 通路
    Lipid metabolism; prostaglandin biosynthesis.
  • 序列相似性
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻译后修饰
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 细胞定位
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

图片

  • ab23672 staining COX2/Cyclooygenase 2 in Human synovial fibroblasts by Flow Cytometry. Cells were detached with accutase, suspended in PBS + 1% BSA, fixed with formaldehyde and permeabilized with methanol for 15 nminutes at -20°C. The sample was incubated with the primary antibody (1/600 in PBS + 1% BSA) for 30 minutes at 22°C. A Biotin-conjugated Rabbit anti-goat polyclonal (1/144) was used as the secondary antibody.
    Gating Strategy: Fibroblasts.
    Legend : Red: isotype; Green: unstimulated; Black: TNF (10 ng/ml for 24 hours) stimulated.

    See Abreview

  • ab23672 at a 1/500 dilution staining COX2 / Cyclooxygenase 2 in human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde and permeabilized using 0.1% Triton X-100. 1% BSA was used to block samples. The secondary used was a Cy5® conjugated rabbit anti-goat at a 1/200 dilution.
  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab23672) at 1/500 dilution

    Lane 1 : Human COX-1
    Lane 2 : Recombinant ovine COX-2 protein
    Lane 3 : Recombinant human COX-2 protein

    Predicted band size: 69 kDa

文献

This product has been referenced in:
  • Lv H  et al. The shear wave elastic modulus and the increased nuclear factor kappa B (NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis. J Biomech 66:44-50 (2018). WB ; Rat . Read more (PubMed: 29137729) »
  • Buckinx R  et al. Expression of corticotropin-releasing factor and urocortins in the normal and Schistosoma mansoni-infected mouse ileum. Cell Tissue Res 359:453-63 (2015). Read more (PubMed: 25358399) »

See all 5 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (peritoneal tumor)
Permeabilization
Yes - Triton-X
Specification
peritoneal tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 24 2018

Application
Western blot
Sample
Human Tissue lysate - whole (Colon)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 15 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon)
Permeabilization
Yes - Triton-x
Specification
Colon
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 15 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Small intestine)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Small intestine
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Small intestine)
Loading amount
50 µg
Specification
Small intestine
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (synovial fibroblasts)
Specification
synovial fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0,1% TritonX-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Torsten Lowin

Verified customer

提交于 Dec 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (synovial fibroblasts)
Specification
synovial fibroblasts
Preparation
Cell harvesting/tissue preparation method: cells detached with accutase (PAA)
Sample buffer: PBS with 1%BSA
Fixation
Formaldehyde
Permeabilization
Yes - methanol, 15min -20 degrees
Gating Strategy
only one gate: fibroblasts
Username

Dr. Torsten Lowin

Verified customer

提交于 Nov 19 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult brain hippocampus)
Specification
adult brain hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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