产品名称Anti-COX1 / Cyclooxygenase 1抗体[5F6/F4]
参阅全部 COX1 / Cyclooxygenase 1 一抗
描述小鼠单克隆抗体[5F6/F4] to COX1 / Cyclooxygenase 1
特异性This antibody recognizes COX1. No cross-reactivity with COX2.
经测试应用适用于: ELISA, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Human
Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
- WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
常规说明Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.001 - 0.01 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
功能May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
通路Lipid metabolism; prostaglandin biosynthesis.
序列相似性Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
细胞定位Microsome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + Huh-7 whole cell lysate at 15 µg
Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution
Performed under reducing conditions.
Observed band size: 68 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST. Gel running conditions: Denaturing. Blocked using 5% milk for 16 hours at 4°C. Detection method: Western lightning chemiluminescent reagent.
Human adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for COX-1 (red) using ab695, with DAPI as nuclear counterstain (blue).
ab695 at 1/400 staining human anal cancer tissue (Left panel) and mouse kidney tissue (Right panel) sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + COX1 protein standard prepared from ram seminal vesicles
COX1 protein standard prepared from ram seminal vesicles supplied by Alpha Diagnostic International.
Lane 1 MM.
Lane 2 Sample
This product has been referenced in:
- Chen WY et al. Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer. Cancer Lett 440-441:35-46 (2019). Read more (PubMed: 30312731) »
- Zhou H et al. Melatonin therapy for diabetic cardiomyopathy: A mechanism involving Syk-mitochondrial complex I-SERCA pathway. Cell Signal 47:88-100 (2018). WB . Read more (PubMed: 29601906) »