Anti-COX1 / Cyclooxygenase 1抗体[5F6/F4] (ab695)
![Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](https://www.abcam.cn/ps/products/0/ab695/Images/ab695-3642-anti-cox1-cyclooxygenase-1-antibody-5f6-f4-flow-cytometry.jpg "Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)")
Key features and details
- Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-COX1 / Cyclooxygenase 1抗体[5F6/F4]
参阅全部 COX1 / Cyclooxygenase 1 一抗 -
描述
小鼠单克隆抗体[5F6/F4] to COX1 / Cyclooxygenase 1 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
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阳性对照
- WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
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常规说明
Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
FCS free -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
5F6/F4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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靶标
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功能
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5742 Human
- Entrez Gene: 19224 Mouse
- Omim: 176805 Human
- SwissProt: P23219 Human
- SwissProt: P22437 Mouse
- Unigene: 201978 Human
- Unigene: 275434 Mouse
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别名
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
图片
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Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
文献 (17)
ab695 被引用在 17 文献中.
- Lim SC et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
- Korczyñska-Krawczyk P et al. The effect of levocetirizine and montelukast on clinical symptoms, serum level and skin expression of COX-1 and COX-2 enzymes in patients suffering from chronic autoimmune urticaria - a pilot study. Postepy Dermatol Alergol 37:73-80 (2020). PubMed: 32467688
- Lim SC et al. Ellagic Acid Inhibits Extracellular Acidity-Induced Invasiveness and Expression of COX1, COX2, Snail, Twist 1, and c-myc in Gastric Carcinoma Cells. Nutrients 11:N/A (2019). PubMed: 31835645
- Chen WY et al. Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer. Cancer Lett 440-441:35-46 (2019). PubMed: 30312731
- Xiao M et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735
