概述

  • 产品名称
    Anti-COX1 / Cyclooxygenase 1抗体[5F6/F4]
    参阅全部 COX1 / Cyclooxygenase 1 一抗
  • 描述
    小鼠单克隆抗体[5F6/F4] to COX1 / Cyclooxygenase 1
  • 宿主
    Mouse
  • 特异性
    This antibody recognizes COX1. No cross-reactivity with COX2.
  • 经测试应用
    适用于: ELISA, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.

  • 阳性对照
    • WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
  • 常规说明
    Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.

性能

应用

Our Abpromise guarantee covers the use of ab695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.001 - 0.01 µg/ml.
WB Use a concentration of 1 - 2 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • 通路
    Lipid metabolism; prostaglandin biosynthesis.
  • 序列相似性
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 细胞定位
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

图片

  • Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + Huh-7 whole cell lysate at 15 µg

    Secondary
    Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size: 68 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST. Gel running conditions: Denaturing. Blocked using 5% milk for 16 hours at 4°C. Detection method: Western lightning chemiluminescent reagent.

    See Abreview

  • Human adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for COX-1 (red) using ab695, with DAPI as nuclear counterstain (blue).

  • ab695 at 1/400 staining human anal cancer tissue (Left panel) and mouse kidney tissue (Right panel) sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

     

    See Abreview

  • Sample: Huh-7 whole cell lysate
    Total protein in input: 1x106 cells
    Treatment: Cells expressing recombitnant myc-tagged COX1
    Immunoprecipitation step: Protein A

    ab695 used at 4 µg/mg lysate, inucbated for 4 hours at 4°C.

    WB antibody was ab9106 used at a 1/5000 dilution.

    See Abreview

  • Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + COX1 protein standard prepared from ram seminal vesicles


    COX1 protein standard prepared from ram seminal vesicles supplied by Alpha Diagnostic International.

    Lane 1 MM.

    Lane 2 Sample

文献

This product has been referenced in:
  • Zhou H  et al. Melatonin therapy for diabetic cardiomyopathy: A mechanism involving Syk-mitochondrial complex I-SERCA pathway. Cell Signal 47:88-100 (2018). WB . Read more (PubMed: 29601906) »
  • Cong R  et al. MicroRNA-218 promotes prostaglandin E2 to inhibit osteogenic differentiation in synovial mesenchymal stem cells by targeting 15-hydroxyprostaglandin dehydrogenase [NAD(+)]. Mol Med Rep 16:9347-9354 (2017). Read more (PubMed: 29039590) »
See all 9 Publications for this product

客户评价及客户问答

1-7 of 7 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CITRATE BUFFER
Sample
Mouse Tissue sections (MOUSE HEART)
Specification
MOUSE HEART
Permeabilization
Yes - 1% TRITON X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (huh-7)
Total protein in input
1e+006 cells
Specification
huh-7
Treatment
cells expressing recombitant myc-tagged COX1
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

提交于 Jun 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cell line)
Loading amount
15 µg
Specification
huh-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Nov 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
200 µg
Specification
293T
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

提交于 Dec 07 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Nov 07 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (anal cancer)
Specification
anal cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Nov 02 2007

Question
Answer

Thank you for your enquiry. I am happy to forward on the reply from the originator of the antibody, which I hope you will find helpful. "Normally IgG antibodies are purified using elution buffers at lower pH of 2-3. During this purification the antibodies are stable and reactive after changing the solution to ph ~ 7. It should be possible to use ab695 under the described conditions." I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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