Anti-COX1 / Cyclooxygenase 1抗体[5F6/F4] (ab695)
Key features and details
- Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-COX1 / Cyclooxygenase 1抗体[5F6/F4]
参阅全部 COX1 / Cyclooxygenase 1 一抗 -
描述
小鼠单克隆抗体[5F6/F4] to COX1 / Cyclooxygenase 1 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
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阳性对照
- WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
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常规说明
Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
FCS free -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
5F6/F4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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靶标
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功能
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5742 Human
- Entrez Gene: 19224 Mouse
- Omim: 176805 Human
- SwissProt: P23219 Human
- SwissProt: P22437 Mouse
- Unigene: 201978 Human
- Unigene: 275434 Mouse
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别名
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
图片
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![Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](https://www.abcam.cn/ps/products/0/ab695/Images/ab695-3642-anti-cox1-cyclooxygenase-1-antibody-5f6-f4-flow-cytometry.jpg)
Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
文献 (15)
ab695 被引用在 15 文献中.
- Lim SC et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
- Chen WY et al. Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer. Cancer Lett 440-441:35-46 (2019). PubMed: 30312731
- Xiao M et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735
- Cianciaruso C et al. Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles. Cell Rep 27:3062-3080.e11 (2019). PubMed: 31167148
- Ding L et al. Downregulation of cyclooxygenase-1 stimulates mitochondrial apoptosis through the NF-?B signaling pathway in colorectal cancer cells. Oncol Rep 41:559-569 (2019). PubMed: 30320345
