Anti-COX IV抗体- Mitochondrial Marker (ab153709)
Key features and details
- Rabbit polyclonal to COX IV - Mitochondrial Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-COX IV抗体- Mitochondrial Marker
参阅全部 COX IV 一抗 -
描述
兔多克隆抗体to COX IV - Mitochondrial Marker -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Monkey -
免疫原
Recombinant fragment within Human COX IV aa 1-169. The exact sequence is proprietary.
Database link: P13073 -
阳性对照
- WB: HepG2 whole cell lysate (ab7900), mouse and rat muscle tissue lysate. IHC-P: Human HS578T xenograft tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab153709于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 19 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
|
说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 19 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the cytochrome c oxidase IV family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Omim: 123864 Human
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
- SwissProt: P10888 Rat
- Unigene: 433419 Human
see all -
别名
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
图片
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Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Mouse muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Rat muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin embedded HS578T (human) xenograft tissue, labeling COX IV using ab153709 at a 1/250 dilution.
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Immunofluorescence analysis of methanol fixed HeLa cells, labeling COX IV using ab153709 at 1/500 dilution (green) and alpha tubulin filaments (red).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab153709 被引用在 7 文献中.
- Wu F & Li C KLF2 up-regulates IRF4/HDAC7 to protect neonatal rats from hypoxic-ischemic brain damage. Cell Death Discov 8:41 (2022). PubMed: 35091544
- Suo D et al. PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes. Exp Ther Med 19:3227-3238 (2020). PubMed: 32269607
- Kowluru RA & Mohammad G Epigenetics and Mitochondrial Stability in the Metabolic Memory Phenomenon Associated with Continued Progression of Diabetic Retinopathy. Sci Rep 10:6655 (2020). PubMed: 32313015
- Lai L et al. Role of endoplasmic reticulum oxidase 1a in H9C2 cardiomyocytes following hypoxia/reoxygenation injury. Mol Med Rep 22:1420-1428 (2020). PubMed: 32626998
- Livingston MJ et al. Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys. Autophagy 15:2142-2162 (2019). PubMed: 31066324
- Gao X et al. Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Mol Med Rep 16:515-522 (2017). IHC ; Rat . PubMed: 28560462
- Panfoli I et al. Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants. FASEB J 30:1416-24 (2016). PubMed: 26655706